APC Mouse Anti-Human IL-4

BD Pharmingen™ APC Mouse Anti-Human IL-4

Name: APC Mouse Anti-Human IL-4
Brand: BD Pharmingen™
SKU: 560671

Clone 8D4-8

(RUO)

Flow cytometric analysis for IL-4 in stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for at least 6 hours with 50 ng/mL PMA (Sigma-Aldrich Cat. No. P-8139) and 250 ng/mL calcium ionophore A23187 (Sigma-Aldrich Cat. No. C-9275) in the presence of 2 µM BD GolgiStop™ (Cat. No. 554724). Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ (Cat. No. 554714) followed by staining with either a APC Mouse IgG1, κ isotype control (left panel) or with the APC Mouse Anti-Human IL-4 antibody (right panel). Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

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제품 세부 정보

Brand: BD Pharmingen™

Product Name: Hu IL-4 APC 8D4-8 50Tst

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Recombinant Human IL-4

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_1727546

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

권장 분석 절차

Flow cytometry: The 8D4-8 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-4 producing cells within mixed cell populations. A useful control investigators may consider using for demonstrating specificity of staining, is to pre-block with one of the following reagents: (1) recombinant human IL-4 (Cat. No. 554605) or (2) unlabeled 8D4-8 antibody (Cat. No. 554515), prior to staining.

제품 고시

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

관련 제품

Fixation/Permeabilization Kit RUO

사이즈 250 Tests 카탈로그 No 554714

Protein Transport Inhibitor (Containing Monensin) RUO

사이즈 0.7 mL 카탈로그 No 554724

APC Mouse IgG1 κ Isotype Control RUO

사이즈 0.1 mg 카탈로그 No 554681

HiCK-2 Human Cytokine Positive Control Cells RUO

사이즈 1 mL 카탈로그 No 555062

Recombinant Human IL-4 RUO

사이즈 5 µg 카탈로그 No 554605

Purified Mouse Anti-Human IL-4 RUO

사이즈 0.5 mg 카탈로그 No 554515

세부 정보보기

560671 Rev. 2

항체 세부 정보

8D4-8

The 8D4-8 monoclonal antibody reacts with human interleukin-4 (IL-4). The immunogen used to raise the 8D4-8 hybridoma was recombinant human IL-4. The 8D4-8 antibody binds to an epitope that is different than the epitope recognized by the MP4-25D2 antibody (Cat. No. 554485).

Clone 8D4-8 displays an increased amount of non-specific binding to dead cells when compared to the clone MP4-25D2. It is recommended to use a fixable viability dye in conjunction with this clone.

560671 Rev. 2

형광 세부 정보

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

560671 Rev.2

인용 및 참고 문헌

개발 참고 자료 (2)

Bird C, Wadhwa M, Thorpe R. Development of immunoassays for human interleukin 3 and interleukin 4, some of which discriminate between different recombinant DNA-derived molecules. Cytokine. 1991; 3(6):562-567. (Biology). 참조 보기
Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). 참조 보기

560671 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.