Flow cytometric analysis of CD4 on Rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD4 (Cat. No. 551980; solid line histogram) or APC Mouse IgG1, κ Isotype Control (Cat. No. 550854; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescence histograms depicting CD4 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.
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Flow cytometric analysis of CD4 on Rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD4 (Cat. No. 551980; solid line histogram) or APC Mouse IgG1, κ Isotype Control (Cat. No. 550854; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescence histograms depicting CD4 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.
제품 세부 정보
BD Pharmingen™
Hu/NHP CD4 APC L200 50Tst
L3T4 ; T-cell surface antigen T4/Leu-3; W3/25 ; CD4 antigen (p55)
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse BALB/c IgG1, κ
Human HPB-ALL Cell Line
Flow cytometry (Routinely Tested)
20 µl
AB_398521
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
준비 및 보관
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
Stain Buffer (FBS) RUO
사이즈
500 mL
카탈로그 No
554656
Stain Buffer (BSA) RUO
사이즈
500 mL
카탈로그 No
554657
Lysing Solution 10X Concentrate IVD
사이즈
100 mL
카탈로그 No
349202
Lysing Buffer RUO
사이즈
100 mL
카탈로그 No
555899
APC Mouse IgG1 κ Isotype Control RUO
사이즈
50 Tests
카탈로그 No
550854
551980 Rev. 6
항체 세부 정보
L200
The L200 monoclonal antibody specifically binds to the human form of the 56 kDa transmembrane glycoprotein, CD4, which is present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. The L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both Rhesus and Cynomolgus Macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) from baboons is also observed. CD4 distribution on lymphocytes is similar for both human and monkey cells, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.
551980 Rev. 6
형광 세부 정보
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
551980 Rev.6
인용 및 참고 문헌
개발 참고 자료 (12)
-
Attanasio R, Dilley D, Buck D, et al. Structural characterization of a cross-reactive idiotype shared by monoclonal antibodies specific for the human CD4 molecule. J Biol Chem. 1991; 266(22):14611-14619. (Biology: Blocking, ELISA, Western blot). 참조 보기
-
Bleavins MR, Brott DA, Alvey JD, de la Iglesia FA. Flow cytometric characterization of lymphocyte subpopulations in the cynomolgus monkey (Macaca fascicularis). Vet Immunol Immunopathol. 1993; 37(1):1-13. (Biology). 참조 보기
-
Giorgi JV, Hultin LE, Desrosiers RC. The immunopathogenesis of retroviral diseases: no immunophenotypic alterations in T, B, and NK cell subsets in SIVmac239-challenged rhesus macaques protected by SIV delta nef vaccination. J Med Primatol. 1996; 25(3):186-191. (Biology). 참조 보기
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Indzhiia LV, Yakovleva LA, Overbaugh J, et al. Baboon T cell lymphomas expressing the B cell-associated surface proteins CD40 and Bgp95. J Clin Invest. 1992; 12(3):225-236. (Biology). 참조 보기
-
Jacobsen CN, Aasted B, Broe MK, Petersen JL. Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet Immunol Immunopathol. 1993; 39(4):461-466. (Biology). 참조 보기
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
-
Powell JD, McClure HM, Anderson D, Fultz PN, Sell KW, Ahmed-Ansari A. Phenotypic and functional differences in NK and LAK cells in the peripheral blood of sooty mangabeys and rhesus macaques. Cell Immunol. 1989; 124(1):107-118. (Biology). 참조 보기
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Savary CA, Lotzova E, Jackson HJ, Jardine JH, Ang KK. Analysis of interleukin-2-activated killer cells of rhesus monkeys: striking resemblance to the human system. J Leukoc Biol. 1993; 54(4):307-313. (Biology). 참조 보기
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Tryphonas H, Lacroix F, Hayward S, Izaguirre C, Parenteau M, Fournier J. Cell surface marker evaluation of infant Macaca monkey leukocytes in peripheral whole blood using simultaneous dual-color immunophenotypic analysis. J Med Primatol. 1996; 25(2):89-105. (Biology). 참조 보기
-
Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. (Biology). 참조 보기
-
Wilson AD, Shooshtari M, Finerty S, Watkins P, Morgan AJ. Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate Saguinus oedipus oedipus (cotton top tamarin). J Immunol Methods. 1995; 178(2):195-200. (Biology). 참조 보기
551980 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
