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- BD® AbSeq Assay
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- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Flow cytometric analysis of Sox2 on human embryonic stem (ES) cells. H7 human ES cells (WiCell, Madison, WI) passage 46 grown on irradiated mouse embryonic fibroblasts were harvested with Accutase™ (Cat No. 561527) and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were permeablized with BD™ Phosflow Perm Buffer III (Cat No. 558050) and stained with Alexa Fluor® 647 Mouse anti-Human Sox2 antibody (solid line) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Clone MOPC-21, Cat. No. 557714, dashed line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Immunoflourescent staining of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 39 grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were permeablized with BD™ Phosflow Perm Buffer III (Cat No. 558050) and stained with Alexa Fluor® 647 Mouse anti-Sox2 monoclonal antibody (pseudo colored red) at 5 µg/mL. Counter-staining of cell nuclei was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Sox2
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Sox2
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제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- mTESR™1 is a trademark of StemCell Technologies.
관련 제품
The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
개발 참고 자료 (3)
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Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). 참조 보기
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Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). 참조 보기
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Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). 참조 보기
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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