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Two-color flow cytometric analysis of CD161a expression on rat splenocytes. Rat splenic leucocytes were stained with FITC Mouse Anti-Rat CD3 antibody (Cat. No. 554832/559975) and either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 565571; Left Panel) or Alexa Fluor® 647 Mouse Anti-Rat CD161a antibody (Cat. No. 565413; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of CD161a (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Rat CD161a
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
관련 제품
The 10/78 monoclonal antibody recognizes the rat CD161 proteins, CD161a (also known as, Klrb1a, or Nkrp1a/NKR-P1A), and CD161b (Klrb1b, Nkrp1b/NKR-P1B). These type II transmembrane glycoproteins have an extracellular C-type lectin domain and thus belong to the C-type lectin superfamily. These CD161 proteins form ~ 60 kDa homodimers that are expressed on natural killer cells and subsets of T lymphocytes, activated monocytes, and dendritic cells. The 10/78 antibody competes with the previously-described 3.2.3 antibody for binding to these CD161 proteins. CD161 molecules are C-type lectin-like receptors that can either activate (CD161a) or inhibit (CD161b) effector leucocyte responses, eg, cytotoxicity or cytokine production, against target cells which express C-type lectin-like related (Clr) molecules. Although several members of the Klrb1 gene family have been identified in the mouse and rat, only a single human KLRB1 homolog has been discovered.
개발 참고 자료 (14)
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Bezouska K, Vlahas G, Horvath O, et al. Rat natural killer cell antigen, NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein. J Biol Chem. 1994; 269(24):16945-16952. (Biology). 참조 보기
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Bezouska K, Yuen CT, O'Brien J, et al. Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity. Nature. 1994; 372(6502):150-157. (Biology). 참조 보기
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Chambers, W.H., Vujanovic, N.L, et al. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. J Exp Med. 1989; 169:1373-1389. (Biology). 참조 보기
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Fujimura T, Yang XF, Soriano R, Ogawa T, Kobayashi M, Jiang H. Cellular surface molecular and cytokine gene expression in rat heart allografts under optimal doses of cyclosporine and FK 506. Transplant Proc. 1998; 30(4):1023-1026. (Clone-specific: Immunohistochemistry). 참조 보기
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Josien R, Heslan M, Soulillou JP, Cuturi MC. Rat spleen dendritic cells express natural killer cell receptor protein 1 (NKR-P1) and have cytotoxic activity to select targets via a Ca2+-dependent mechanism. J Exp Med. 1997; 186(3):467-472. (Biology). 참조 보기
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Kirkham CL, Carlyle JR. Complexity and Diversity of the NKR-P1:Clr (Klrb1:Clec2) Recognition Systems. Front Biosci. 2014; 5(5):1-16. (Clone-specific). 참조 보기
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Kraus E, Lambracht D, Wonigeit K, Hunig T. Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996; 26(11):2582-2586. (Clone-specific: Flow cytometry, Immunoprecipitation). 참조 보기
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Lanier LL. Natural killer cells: from no receptors to too many. Immunity. 1997; 6(4):371-378. (Biology). 참조 보기
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Li J, Rabinovich BA, Hurren R, Shannon J, Miller RG. Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B. Int Immunol. 2003; 15(3):411-416. (Clone-specific: Activation, Calcium Flux, Cytotoxicity, Flow cytometry, Functional assay, Inhibition). 참조 보기
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Ryan JC, Niemi EC, Nakamura MC, Seaman WE. NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity. J Exp Med. 1995; 181(5):1911-1915. (Biology). 참조 보기
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Scriba A, Grau V, Steiniger B. Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43. Scand J Immunol. 1998; 47(4):332-342. (Biology). 참조 보기
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Scriba A, Schneider M, Grau V, van der Meide PH, Steiniger B. Rat monocytes up-regulate NKR-P1A and down-modulate CD4 and CD43 during activation in vivo: monocyte subpopulations in normal and IFN-gamma-treated rats. J Leukoc Biol. 1997; 62(6):741-752. (Biology). 참조 보기
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Trinite B, Voisine C, Yagita H, Josien R. A subset of cytolytic dendritic cells in rat. J Immunol. 2000; 165(8):4202-4208. (Biology). 참조 보기
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Webster GA, Bowles MJ, Karim MS, Wood RF, Pockley AG. Activation antigen expression on peripheral blood neutrophils following rat small bowel transplantation. NKR-P1 is a novel antigen preferentially expressed during allograft rejection. Transplantation. 1994; 58(6):707-712. (Biology). 참조 보기
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