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Alexa Fluor® 647 Mouse Anti-Human FCRL5/FCRL3 (CD307e/c)
제품 세부 정보
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BD Pharmingen™
FCRL5/FCRL3; FcRH5/FcRH3; IRTA2/IRTA3
Human (QC Testing)
Mouse IgG2b, κ
Human FCLR5 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl/test
AB_2869565
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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제품 고시

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. An isotype control should be used at the same concentration as the antibody of interest.
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564343 Rev. 1
항체 세부 정보
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307307

The 307307 monoclonal antibody specifically binds to Fc receptor-like 5 (FCLR5) and crossreacts with Fc receptor-like 3 (FCLR3). These type I transmembrane glycoproteins have sequences that are homologous to classical Fcγ receptors. FCLR5 is also known as CD307e, FCRH5 (Fc receptor homolog 5) and IRTA2 (immunoglobulin superfamily receptor translocation associated 2) whereas FCLR3 is referred to as CD307c, FCRH3 (Fc Receptor Homolog 3) and IRTA3 (immunoglobulin superfamily receptor translocation associated 3). FCRL5 and FCRL3 are differentially expressed by mature B cell subsets and play key roles in controlling signaling through the B cell receptor.

564343 Rev. 1
형광 세부 정보
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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Alexa Fluor™ 647
653 nm
669 nm
564343 Rev.1
인용 및 참고 문헌
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View product citations for antibody "564343" on CiteAb

개발 참고 자료 (8)

  1. Ehrhardt GR, Cooper MD. Immunoregulatory roles for fc receptor-like molecules. Curr Top Microbiol Immunol. 201; 350:89-104. (Biology). 참조 보기
  2. Franco A, Damdinsuren B, Ise T, et al. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. J Immunol. 2013; 190(11):5739-5746. (Biology). 참조 보기
  3. Hatzivassiliou G, Miller I, Takizawa J, et al. IRTA1 and IRTA2, novel immunoglobulin superfamily receptors expressed in B cells and involved in chromosome 1q21 abnormalities in B cell malignancy. Immunity. 2001; 14(3):277-289. (Biology). 참조 보기
  4. Kochi Y, Myouzen K, Yamada R, et al. FCRL3, an autoimmune susceptibility gene, has inhibitory potential on B-cell receptor-mediated signaling. J Immunol. 2009; 183(9):5502-5510. (Biology). 참조 보기
  5. Maltais LJ, Lovering RC, Taranin AV, et al. New nomenclature for Fc receptor-like molecules. Nat Immunol. 2006; 7(5):431-432. (Biology). 참조 보기
  6. Nagata S, Ise T, Pastan I. Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009; 182(12):7518-7526. (Biology). 참조 보기
  7. Swainson LA, Mold JE, Bajpai UD, McCune JM. Expression of the autoimmune susceptibility gene FcRL3 on human regulatory T cells is associated with dysfunction and high levels of programmed cell death-1. J Immunol. 2010; 184(7):3639-3647. (Biology). 참조 보기
  8. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012; 188(10):4741-4745. (Biology). 참조 보기
모두 보기 (8) 간단히 보기
564343 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.