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Multiparameter flow cytometric analysis of CLEC5A (MDL-1) expression on human peripheral blood leucocytes populations. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leukocytes were washed, incubated with Human BD Fc Block™ (Cat. No. 564219/564220), and then stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CLEC5A (MDL-1) antibody (Cat. No. 566585; Right Plot) at 0.25 μg/test. Two-parameter flow cytometric contour plots showing the correlated expression of CLEC5A (MDL-1) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. A similar staining profile was observed when human blood was stained first followed by erythrocyte lysis with BD Pharm Lyse™ Lysing Buffer. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CLEC5A (MDL-1)
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준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
Monoclonal antibody 283834 specifically recognizes C-type lectin domain family 5, member A (CLEC5A) which is also known as C-type lectin superfamily member 5 (CLECSF5) or Myeloid DAP12-associating Lectin-1 (MDL-1). CLEC5A (MDL-1) is a ~40 kDa type II transmembrane glycoprotein that is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, and neutrophils. Clec5A (MDL-1) has a charged lysine in its transmembrane region that allows it to associate with DAP12 (aka, TYROBP) and transduce an activating signal in myeloid cells. Clec5A (MDL-1) regulates inflammatory responses during autoimmune arthritis and upon binding to Dengue viruses. The 283834 antibody does not crossreact with recombinant mouse Clec5a (MDL1) protein.
개발 참고 자료 (5)
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Bakker AB, Baker E, Sutherland GR, Phillips JH, Lanier LL. Myeloid DAP12-associating lectin (MDL)-1 is a cell surface receptor involved in the activation of myeloid cells.. Proc Natl Acad Sci USA. 1999; 96(17):9792-6. (Biology). 참조 보기
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Chen ST, Liu RS, Wu MF, et al. CLEC5A regulates Japanese encephalitis virus-induced neuroinflammation and lethality.. PLoS Pathog. 2012; 8(4):e1002655. (Biology). 참조 보기
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González-Domínguez É, Domínguez-Soto Á, Nieto C, et al. Atypical Activin A and IL-10 Production Impairs Human CD16+ Monocyte Differentiation into Anti-Inflammatory Macrophages.. J Immunol. 2016; 196(3):1327-37. (Biology). 참조 보기
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Watson AA, Lebedev AA, Hall BA, et al. Structural flexibility of the macrophage dengue virus receptor CLEC5A: implications for ligand binding and signaling.. J Biol Chem. 2011; 286(27):24208-18. (Biology). 참조 보기
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Wortham BW, Eppert BL, Flury JL, et al. Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies.. J Immunol. 2016; 196(8):3227-31. (Biology). 참조 보기
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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