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권장 분석 절차
An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry).
We recommend PE-conjugated mouse IgG1 κ mAb MOPC-21 (Cat. No. 555749) for immunofluorescent staining of human whole blood, mAb MOPC-21 (Cat. No. 554680 or 559320) for intracellular cytokine flow cytometry, and mAb MOPC-21 (Cat. No. 556650) for non-human primate cells.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The MOPC-31C antibody has unknown specificity. The transplantable plasmacytoma MOPC-31C was induced by intraperitoneal injection of mineral oils into BALB/c mice. It was adapted to continuous cell culture by alternate passage in animals.
개발 참고 자료 (2)
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Hay R, Caputo J, Chen TR, Macy M, McClintock P, Reid Y, ed. ATCC. Cell Lines and Hybridomas, Eighth Edition. 1994:75.
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Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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