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      PE Mouse Anti-Human P-glycoprotein
      제품 세부 정보
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      BD™
      Anti-P-glycoprotein PE 15D3 50TstRUO/GMP
      ABCB1; ABC20; CLCS; GP170; MDR1; P-GP; PGY1; pgp 170
      Human
      Mouse BALB/c IgG1, κ
      MDR1-transfected BATV.2 cells
      Flow cytometry
      20 μL
      5243
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      준비 및 보관

      The PE conjugate is supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340555 Rev. 1
      항체 세부 정보
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      15D3

      Anti–P-glycoprotein (P-gp), clone 15D3, is generated from the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a BA3T3 fibroblast line (BATV.2) transfected with the human MDR1 gene.

      Anti–P-glycoprotein (P-gp) recognizes a 170-kdalton (kDa) protein from multidrug resistant (MDR) cells.

      340555 Rev. 1
      형광 세부 정보
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340555 Rev.1
      인용 및 참고 문헌
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      개발 참고 자료 (10)

      1. Akolkar PN, Gulwani-Akolkar B, Pergolizzi R, Bigler RD, Silver J. Influence of HLA genes on T cell receptor V segment frequencies and expression levels in peripheral blood lymphocytes. J Immunol. 1993; 150(7):2761-2773. (Biology). 참조 보기
      2. Beck WT, Grogan TM, Willman CL, et al. Methods to detect P-glycoprotein–associated multidrug resistance in patients tumors: concensus recommendations. Cancer Res. 1996; 56:3010-3020. (Biology).
      3. Chan HS, Haddad G, Zheng L, Bradley G, Dalton WS, Ling V. Sensitive immunofluorescence detection of the expression of P-glycoprotein in malignant cells.. Cytometry. 1997; 29(1):65-75. (Biology). 참조 보기
      4. Dalton WS, Durie BG, Alberts DS, Gerlach JH, Cress AE. Characterization of a new drug-resistant human myeloma cell line that expresses P-glycoprotein.. Cancer Res. 1986; 46(10):5125-30. (Biology). 참조 보기
      5. Drach D, Zhao S, Drach J, et al. Subpopulations of normal peripheral blood and bone marrow cells express a functional multidrug resistant phenotype.. Blood. 1992; 80(11):2729-34. (Biology). 참조 보기
      6. Filipits M, Suchomel RW, Dekan G, et al. MRP and MDR1 gene expression in primary breast carcinomas.. Clin Cancer Res. 1996; 2(7):1231-7. (Biology). 참조 보기
      7. Gupta S, Aggarwal S. P-glycoprotein in cells of the human immune system. The Immunologist 4/3. 1996; 86-90. (Biology).
      8. Klimecki WT, Futscher BW, Grogan TM, Dalton WS. P-glycoprotein expression and function in circulating blood cells from normal volunteers.. Blood. 1994; 83(9):2451-8. (Biology). 참조 보기
      9. List AF. Role of multidrug resistance and its pharmacological modulation in acute myeloid leukemia.. Leukemia. 1996; 10(6):937-42. (Biology). 참조 보기
      10. Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). 참조 보기
      모두 보기 (10) 간단히 보기
      340555 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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