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- BD® AbSeq Assay
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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BD Oncomark™ Anti-Human TCR-αβ FITC/TCR-γδ PE/CD3 PerCP-Cy™5.5
(RUO (GMP))Anti-Human TCR-αβ FITC/TCR-γδ PE/CD3 PerCP-Cy™5.5
규제 상태 범례
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설명
Anti–TCR-α/β-1, clone WT31, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. Anti–TCR-γ/δ-1, clone 11F2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a Sepharose® bead/CD3/γ/δ TCR complex. CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. Anti–TCR-α/β-1 recognizes a conformational epitope formed by the T-cell receptor (TCR) for antigen and the CD3 epsilon chain. The α/β TCR is a disulfide-linked 80-kilodalton (kd) heterodimer consisting of a 44-kd α chain and a 37-kd β chain. Anti–TCR-γ/δ-1 reacts with a framework epitope of the γ/δ T-cell antigen receptor (TCR). The γ/δ TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non–disulfide-linked heterodimers with the δ subunit. CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).
준비 및 보관
Store vials at 2° to 8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
설명 | Clone | Isotype | EntrezGene ID |
---|---|---|---|
gamma delta TCR PE | 11F2 | IgG1, | N/A |
CD3 PerCP-Cy5.5 | SK7 | IgG1, κ | N/A |
TCR αβ FITC | WT31 | IgG1, κ | N/A |
개발 참고 자료 (20)
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Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Biology). 참조 보기
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Brenner M, Groh V, Porcelli S, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:1049-1053.
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Brenner MB, Groh V, Porcelli SA, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1986:145-149.
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Campana D, Coustan-Smith E, Janossy G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:297-298.
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Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
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Falini B, Flenghi L, Fagioli M, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:303-305.
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Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Biology). 참조 보기
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Lanier LL, Serafini AT, Ruitenberg JJ, et al. The γ T-cell antigen receptor. J Clin Immunol. 1987; 7:429-440. (Biology).
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Lanier LL, Weiss A. Presence of Ti (WT31) negative T lymphocytes in peripheral blood and thymus. Nature. 1986; 324:268. (Biology).
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Ledbetter JA, Uckun FM. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:312-314.
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Matsuo Y, Ariyasu T, Brenner MB, Imanishi J, Yokoyama MM, Minowada J. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:299-301.
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Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). 참조 보기
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Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 참조 보기
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Slameron A, Sanchez-Madrid F, Ursa MA, Fresno M, and Alarcon B. A conformational epitope expressed upon association of the CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991; 147:3047-3052. (Biology).
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Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen.. J Immunol. 1985; 135(3):1922-8. (Biology). 참조 보기
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Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor γ/δ-bearing T lymphocytes. Eur J Immunol. 1989; 19:185-188. (Biology).
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Weiss A, Newton M, Crommie D. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer.. Proc Natl Acad Sci USA. 1986; 83(18):6998-7002. (Biology). 참조 보기
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van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
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