PerCP-Cy™5.5 Mouse Anti-Human CD22

BD™ PerCP-Cy™5.5 Mouse Anti-Human CD22

Name: PerCP-Cy™5.5 Mouse Anti-Human CD22
Brand: BD™
SKU: 658329

Clone S-HCL-1 (also known as S-HCL1)

(RUO (GMP))

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제품 세부 정보

Brand: BD™

Product Name: CD22 PerCP-Cy5.5 (S-HCL-1) RUO

Alternative Name: SIGLEC2; SIGLEC-2; Bgp135; BL-CAM; Lyb8; LPAP

Reactivity: Human

Isotype: Mouse IgG2b, κ

Immunogen: Hairy Cell Leukemia Cells and Membranes

Application: Flow cytometry

Concentration: 3 µg/mL

Vol. Per Test: 20 μL

Workshop Number: II B49

Entrez Gene ID: 933

Regulatory Status: RUO (GMP)

658329 Rev. 1

항체 세부 정보

S-HCL-1

The CD22 antibody, clone S-HCL-1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from CD-1 mice immunized with whole hairy cell leukemia cells and membrane preparations derived from them.

The CD22 antibody recognizes a 135-kilodalton (kDa) type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD22 antigen is also known as BL-CAM, Bgp135, and Siglec2.

658329 Rev. 1

형광 세부 정보

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

658329 Rev.1

인용 및 참고 문헌

개발 참고 자료 (13)

Borowitz MJ, Bousvaros A, Brynes RK, et al. Monoclonal antibody phenotyping of B-cell non-Hodgkin's lymphomas. The Southeastern Cancer Study Group experience.. Am J Pathol. 1985; 121(3):514-21. (Biology). 참조 보기
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Doody GM, Dempsey PW, Fearon DT. Activation of B lymphocytes: integrating signals from CD19, CD22 and Fc γ RIIb1. Curr Opin Immunol. 1996; 8:378-382. (Biology).
Foon KA, Gale RP, Todd RF. Recent advances in the immunologic classification of leukemia.. Semin Hematol. 1986; 23(4):257-83. (Biology). 참조 보기
Law CL, Sidorenko SP, Clark EA. Regulation of lymphocyte activation by the cell-surface molecule CD22. Immunol Today. 1994; 15(9):442-449. (Biology). 참조 보기
Loken MR, Shah VO, Hollander Z, Civin CI. Flow cytometric analysis of normal B lymphoid development.. Pathol Immunopathol Res. 1988; 7(5):357-70. (Biology). 참조 보기
Mason DY, Stein H, Gerdes J, et al. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid. Blood. 1987; 836-840. (Biology).
Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). 참조 보기
Terstappen LW, Johnsen S, Segers-Nolten IM, Loken MR. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry.. Blood. 1990; 76(9):1739-47. (Biology). 참조 보기
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). 참조 보기

모두 보기 (13)

658329 Rev. 1

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