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The CD64 antibody, clone 10.1, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from a BALB/c mouse that was immunized first with 2 x 107 rheumatoid synovial fluid cells and on subsequent occasions with 1.5 x 107 fibronectin-purified human monocytes obtained from pools of blood group–matched donors.
CD64 recognizes the 72-kilodalton (kDa) human FcγRI that can bind monomeric IgG.
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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