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CD57 Biotin
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BD™
CD57 Biotin HNK-1 ASR
Human
Mouse IgM, κ
Membrane extracts of the HSB-2 T-lymphoblastoid cell line
Flow cytometry
12.5 μg/mL
20 μL
27087
Phosphate buffered saline with gelatin and 0.1% sodium azide.
ASR


준비 및 보관

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340861 Rev. 1
항체 세부 정보
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HNK-1

The CD57 antibody, clone HNK-1, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with lymph node cells isolated from BALB/c mice immunized with membrane extracts of the HSB-2 T–lymphoblastoid cell line.

The CD57 (Leu-7) antibody recognizes a 110-kilodalton (kDa) glycoprotein that is expressed on a subset of natural killer (NK) lymphocytes and T lymphocytes. The antigen is a carbohydrate structure associated with myelin-associated glycoprotein.

340861 Rev. 1
형광 세부 정보
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
340861 Rev.1
인용 및 참고 문헌
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개발 참고 자료 (10)

  1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981; 127(3):1024-1029. (Biology). 참조 보기
  2. Bunn PA, Gazdar AF, Carney DN, Minna J. Small cell lung carcinoma and natural killer cells share an antigenic determinant, Leu-7. Clin Res. 1984; 32:416A. (Biology).
  3. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Kruse J, Mailhammer R, Wernecke H, et al. Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1.. Nature. 1984 September; 311(5982):153-155. (Biology). 참조 보기
  6. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 참조 보기
  7. Manara GC, De Panfilis G, Ferrari C.. Ultrastructural characterization of human large granular lymphocyte subsets defined by the expression of HNK-1 (Leu-7), Leu-11, or both HNK-1 and Leu-11 antigens.. J Histochem Cytochem. 1985 November; 33(11):1129-1133. (Biology). 참조 보기
  8. McGarry RC, Helfand SL, Quarles RH, Roder JC. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature. 1983; 306(5941):376-378. (Biology). 참조 보기
  9. Mechtersheimer G, Staudter M, Möller P.. Expression of the natural killer cell-associated antigens CD56 and CD57 in human neural and striated muscle cells and in their tumors.. Cancer Res. 1991 February; 51(4):1300-1307. (Biology). 참조 보기
  10. Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:711-714.
모두 보기 (10) 간단히 보기
340861 Rev. 1

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