CD22 (S-HCL-1) PE-Cy7

BD™ CD22 (S-HCL-1) PE-Cy7

Name: CD22 (S-HCL-1) PE-Cy7
Brand: BD™
SKU: 664538

Clone S-HCL-1 (also known as S-HCL1)

(ASR)

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제품 세부 정보

Brand: BD™

Product Name: CD22 (S-HCL-1) PE-Cy7 ASR

Alternative Name: SIGLEC2; SIGLEC-2; Bgp135; BL-CAM; Lyb8; LPAP

Reactivity: Human

Isotype: Mouse IgG2b, κ

Immunogen: Hairy Cell Leukemia Cells and Membranes

Application: Flow cytometry

Vol. Per Test: 5 µl/test

Workshop Number: II B49

Entrez Gene ID: 933

Storage Buffer: Phosphate buffered saline with BSA and 0.1% sodium azide.

Regulatory Status: ASR

664538 Rev. 1

항체 세부 정보

S-HCL-1

The CD22 antibody, clone S-HCL-1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from CD-1 mice immunized with whole hairy cell leukemia cells and membrane preparations derived from them.

The CD22 antibody recognizes a 135-kilodalton (kDa) type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD22 antigen is also known as BL-CAM, Bgp135, and Siglec2.

664538 Rev. 1

형광 세부 정보

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE-Cy7

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

664538 Rev.1

인용 및 참고 문헌

개발 참고 자료 (11)

Borowitz MJ, Bousvaros A, Brynes RK, et al. Monoclonal antibody phenotyping of B-cell non-Hodgkin's lymphomas. The Southeastern Cancer Study Group experience.. Am J Pathol. 1985; 121(3):514-21. (Biology). 참조 보기
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Doody GM, Dempsey PW, Fearon DT. Activation of B lymphocytes: integrating signals from CD19, CD22 and Fc γ RIIb1. Curr Opin Immunol. 1996; 8:378-382. (Biology).
Law CL, Sidorenko SP, Clark EA. Regulation of lymphocyte activation by the cell-surface molecule CD22. Immunol Today. 1994; 15(9):442-449. (Biology). 참조 보기
Loken MR, Shah VO, Hollander Z, Civin CI. Flow cytometric analysis of normal B lymphoid development.. Pathol Immunopathol Res. 1988; 7(5):357-70. (Biology). 참조 보기
Mason DY, Stein H, Gerdes J, et al. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid. Blood. 1987; 836-840. (Biology).
Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). 참조 보기
Terstappen LW, Johnsen S, Segers-Nolten IM, Loken MR. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry.. Blood. 1990; 76(9):1739-47. (Biology). 참조 보기
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).

모두 보기 (11)

664538 Rev. 1

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