CD19 PerCP

BD™ CD19 PerCP

Name: CD19 PerCP
Brand: BD™
SKU: 340721

Clone SJ25C1 (also known as SJ25-C1)

(ASR)

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Scientific Resources

제품 세부 정보

Brand: BD™

Product Name: CD19 PerCP SJ25C1 ASR

Alternative Name: B4; B-lymphocyte antigen CD19; Leu-12

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human NALM1 + NALM16 cells

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Workshop Number: II L17; III 073

Entrez Gene ID: 930

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

준비 및 보관

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340721 Rev. 1

항체 세부 정보

SJ25C1

The CD19 antibody, clone SJ25C1, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with NALM1 and NALM16 cells.

The CD19 antibody recognizes a 90-kilodalton (kDa) antigen that is present on human B lymphocytes.

340721 Rev. 1

형광 세부 정보

PerCP

PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PerCP

Excitation Source: Blue 488 nm

Excitation Max: 481 nm

Emission Max: 675 nm

340721 Rev.1

인용 및 참고 문헌

개발 참고 자료 (6)

Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. KnappW, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York,NY: Oxford University Press; 1989:46-48.
Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). 참조 보기
Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 참조 보기
Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). 참조 보기

모두 보기 (6)

340721 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.