Anti-TCRγ/δ-1 PE-Cy™7

BD™ Anti-TCRγ/δ-1 PE-Cy™7

Name: Anti-TCRγ/δ-1 PE-Cy™7
Brand: BD™
SKU: 655434

Clone 11F2

(ASR)

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Scientific Resources

제품 세부 정보

Brand: BD™

Product Name: TCR gamma/delta-1 PE-Cy7 (11F2) ASR

Alternative Name: TCRgd; γδ TCR; TRG@/TRD@; TCRG/TCRD; TCR gamma delta

Reactivity: Human

Isotype: Mouse BALB/c IgG1

Immunogen: Sepharose® bead/CD3/γ/δ TCR complex

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 5 μL

Entrez Gene ID: 6964, 6965

Storage Buffer: Phosphate buffered saline with BSA and 0.1% sodium azide.

Regulatory Status: ASR

준비 및 보관

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

655434 Rev. 1

항체 세부 정보

11F2

The Anti–TCRγ/δ-1 antibody, clone 11F2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a Sepharose® bead/CD3/γ/δ TCR complex.

The Anti–TCRγ/δ-1 antibody reacts with a framework epitope of the γ/δ T-cell antigen receptor (TCR). The γ/δ TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non–disulfide-linked heterodimers with the δ-subunit.

655434 Rev. 1

형광 세부 정보

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE-Cy7

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

655434 Rev.1

인용 및 참고 문헌

개발 참고 자료 (6)

Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Biology). 참조 보기
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Biology). 참조 보기
Lanier LL, Serafini AT, Ruitenberg JJ, et al. The γ T-cell antigen receptor. J Clin Immunol. 1987; 7:429-440. (Biology).
Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor γ/δ-bearing T lymphocytes. Eur J Immunol. 1989; 19:185-188. (Biology).

모두 보기 (6)

655434 Rev. 1

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