Cell Biology
Sample Preparation
Immunoprecipitation
Immunoprecipitation with Antibody: Agarose Conjugates
Find protocols for cell lysate preparation under denaturing and native conditions, and step-by-step instructions for immunoprecipitation of antibody:agarose conjugates.
Immunoprecipitation with anti-Phosphotyrosine: Biotin Conjugates
Find protocols for cell lysate preparation under denaturing and native conditions, and step-by-step instructions for immunoprecipitation of anti-PTyr:Biotin complexes.
Immunoprecipitation with Soluble Antibodies
Find protocols for tissue sectioning and immunohistochemical staining of frozen sections.
Western Blotting
Western Blotting with Rabbit Polyclonal Antibodies
Find complete protocol for western blotting with rabbit polyclonal antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.
Western Blotting with Monclonal Antibodies
Get complete protocol for western blotting with monoclonal antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.
Western Blotting with Biotinylated Antibodies
Find complete protocol for western blotting with biotinylated antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.
Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
Find complete protocol for western blotting with biotinylated antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.
Imaging
Bioimaging in 96 -well plates
Get step-by-step instructions from sample preparation to staining for bioimaging of cells.