BD FACSymphony™ Flow Cytometer

High parameter in a smaller footprint

The BD FACSymphony™ A3 Cell Analyzer allows for flexibility to match your needs and budgets. Configure with up to five lasers and 30 parameters enabling up to 28 colors simultaneously. Lasers and detectors can also be implemented later with field upgrades.

Lasers

Select up to five lasers for optimization of your research needs. Configure your instrument using traditional and/or unique wavelengths and power ratings.

Optical arrays

Photomultiplier tubes (PMTs) in decagon arrays support up to 10 detectors.

Filter sets are exchangeable for traditional fluorescent markers, as well as specialized sets for unique dyes or proteins.

More choices, more sensitivity

Lasers

Select up to nine lasers for maximum flexibility and future-proofing for new lasers, dyes and applications to come. Configure your instrument to meet your research needs using traditional and/or unique wavelengths and power ratings.

Optical arrays

Offering more flexibility than ever, choose from

• Classic tube photomultiplier tubes (PMTs) in decagon arrays with up to 10 detectors
• Small square form PMT and GaAs/GaAsP* detectors in high-parameter cascade arrays (HPC)
  • Detectors optimized for increased sensitivity and lower noise in ranges of the spectrum
  • Up to 20 detectors per laser
  • Discrete pre-amplifiers

Filter sets are exchangeable for traditional fluorescent markers, as well as specialized sets for unique dyes or proteins.

BD offers a broad portfolio of high-quality conjugate combinations to deliver flexibility in panel design and accelerate time to insight

BD offers a vast portfolio of innovative, high-quality reagents to expand experimental design and help accelerate time to insight. BD is the exclusive provider of BD Horizon Brilliant™ Fluorochromes, which are based on Nobel Award-Winning Sirigen polymer technology and offer greater resolving power than traditional dyes. BD is committed to the development of new fluorochromes across existing and new laser lines to support researchers and ultimately advance science.

Custom reagents are also available through the BD Custom Reagents Program to support high-parameter panel optimization across various areas of research. For more information visit our Custom Reagents page or contact BDB_HPS_VIP@bd.com.

CD4 fluorochrome evaluation kits

Each BD FACSymphony™ Cell Analyzer comes with reagents that will be used for on-site training and instrument characterization. Each BD fluorochrome optimized for flow cytometry has been conjugated to anti-human or mouse CD4 and is provided for PMT voltrations and instrument characterization. Mid-and low-expressed antigen specificities are also available. 

Representative PMTV titration and measured Stain Index of PBMCs stained with clone SK3 mouse anti-human CD4 acquired at varying PMTVs in 25 V increments. Stain index and resolution for this detector is nearing maximal at a PMTV of 425 V and above as indicated by the square.

Optimizing reagents for panel design

Speak with your representative about including reagents as part of your instrument purchase to minimize the cost and risk of optimizing new high-parameter panels on your BD FACSymphony™ Cell Analyzer. Our high-parameter specialists will work with you to identify the optimal panel for your experimental design and instrument configuration and provide the reagents needed to get to the final rendition of your panel.

Accelerate your discovery

High-dimensional data analysis

Advancements in data analysis algorithms enable us to visualize and identify cell populations in more meaningful ways. With high-dimensional data of up to 50 parameters, this has never been more important to accelerate discovery.

Take your analysis to the next level with new features in FlowJo™ Software, v10 including improved BD FACSDiva™ Software support, platform overlays, spectral compensation and more. Keep up on the latest plug-ins for high-dimensional visualization and analysis at FlowJo Exchange.