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Overview

Next-generation laser-specific flow cytometry research reagents

The family of BD Horizon RealYellow™ and RealBlue™ Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

 

Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

 
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RB780
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BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7

RB780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser to offer.

 

  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Bright fluorochrome to support the detection of low-expression surface and intracellular markers 
  • Reduced monocyte background compared to PE-Cy7

 

Download the BD Horizon RealBlue™ 780 Reagents Brochure >

 
RB-780 video
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RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers

 

RB780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.

Performance

BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7

RB-780 Emission Profile
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Normalized emission profile of PE-Cy7 and RB780 with RB780 minimal excitation off the 561-nm yellow-green laser.

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Human whole blood was stained with BD Horizon™  RB780 Human CD4 (SK3) Reagent, acquired and compensated on a BD FACSymphony  A5 SE Cell Analyzer.

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BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

Applications

BD Horizon RealBlue™ 780 Regents are compatible with a broad range of fixation and permeabilization systems

 

Buffers Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer Compatible
CellBlox™ Blocking Buffer Compatible
BD Cytofix™ Fixation Buffer Stable at least 24 hours
1% PFA Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and permeabilization Solution Compatible with antibody staining before and after fixation
BD FACS™ Permeabilization Solution II Compatible with antibody staining before and after fixation
BD Phosflow™ Perm III Buffer Compatible with antibody staining before and after fixation
EDTA and Heparin Compatible
BD Horizon™ Brilliant Stain Buffer (BSB) Compatible
 
RY586
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Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents

BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:

 

  • Minimal cross-laser excitation off the 488-nm blue laser
  • Bright fluorescence to detect low-expression surface and intracellular markers 

 

Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.

 

Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.

 
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Generate reliable data that clearly resolve even the most challenging cell populations by leveraging bright, clean fluorochromes that minimize spillover and background.
Maximize panel design options for both conventional and spectral flow cytometry by using reagents that deliver minimal cross-laser excitation in hundreds of antibody specificities.
Reveal more insights from the same sample by expanding the utility of blue and yellow-green laser channels with reagents that enable the simultaneous use of more detectors.

Performance

Click through the slides below to view performance data.

 

 

Fill in this form to receive your BD Horizon RealBlue™ 780 sample


Take a step closer to maximizing panel flexibility for spectral flow cytometry



 

   

   

BD flow cytometers are Class 1 Laser Products.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

CellBlox is a trademark of Thermo Fisher Scientific.

 

Terms and Conditions

  1. Offer valid from November 24th 2022 to March 31st 2023
  2. Cannot be combined with any other offer
  3. Valid in participating countries onlly
  4. Valid once per user
  5. Offer valid on BD Horizon RealBlue™ 780 25 test/ug size only
  6. There are a limited number of samples available, and BD retains the right to cancel this offer at any time

 

For Research Use Only. Not for use in diagnostic or therapeutic procedures.