This page has been recently translated and is available in French now.
Spend Less Time Optimizing Panels and More Time Discovering
The family of BD Horizon RealYellow™ and RealBlue™ Reagents, with bright, laser-specific fluorochromes and a wide array of antibody specificities, can help streamline the path to your scientific discoveries.
Simplify panel design and improve data resolution even for the most complex analysis with a new family of spectrally optimized fluorochromes for the blue and yellow-green laser lines.
Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover and laser-precision to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.
BD Horizon RealYellow™ 586 (RY586) Reagents
BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:
- Minimal cross-laser excitation off the 488-nm blue laser
- Bright fluorescence to detect low-expression surface and intracellular markers
Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.
Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.
Click through the slides below to view performance data.
Clean & Bright
BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation
BD Horizon RealYellow™ 586 Reagents exhibit minimal cross-laser excitation off the blue laser, resulting in significantly less spillover into blue detectors than PE. At the same time, they offer minimal background and high resolution comparable to PE.
BD Horizon RealYellow™ 586 Reagents have versatile applications
RY586 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RY586 reagents can be used instead of PE on conventional flow cytometers or with PE on spectral flow cytometers equipped with the 561-nm yellow-green laser to meet your specific panel design needs.
Spectral Flow Cytometry
BD Horizon RealYellow™ Reagents can be used with spectral flow cytometry
RY586 can be used with PE on spectral flow cytometers. Since RY586 provides comparable brightness to PE, it is ideal for detecting low-expression markers. Using RY586 and PE in the same spectral flow cytometry panel enables high-dimensional analysis of more low-expression surface and intracellular markers.
Stable & Consistent
Performance stability of BD Horizon RealYellow™ Reagents RY586 offers stable reagent performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents as well as proven photostability when exposed to typical lab lighting or dimmed core lab lighting.
RY586 made-to-stock reagents and BD OptiBuild™ On-Demand Reagents show consistent performance between each other and across multiple lots.
Human whole blood was stained with human CD4 (SK3) RY586, followed by lysis with BD FACS™ Lysing Solution. Mouse splenocytes were stained with mouse CD8a 53-6.7. All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.
BD Horizon RealYellow™ Reagents are compatible with a variety of common fixation and permeabilization systems, including:
- BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit
- BD Pharmingen™ Transcription Factor Buffer Set
- BD FACS™ Lysing Solution
- BD Pharm Lyse™ Lysing Buffer
- BD Cytofix™ Fixation Buffer
- BD FACS™ Permeabilizing Solution 2
- BD Phosflow™ Perm III
- BD Horizon™ Brilliant Stain Buffer (BSB)
- 1% PFA
- EDTA and heparin
- CellBlox™ Blocking Buffer
Start a conversation.
Request a fluorochrome sample or panel design consultation.
- Product Information Sheet
BD flow cytometers are Class 1 Laser Products.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CellBlox is a trademark of Thermo Fisher Scientific.