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BD Horizon RealYellow™
and BD Horizon RealBlue™ Reagents

Overview

Next-generation laser-specific flow cytometry research reagents


The family of BD Horizon RealYellow Reagents and BD Horizon RealBlue Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

 

Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimise spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimise resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

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    Still Using PerCP- and PE-Based Tandem Dyes? Time for an Upgrade.

    FormatSpectralConventionalRelative BrightnessSpillover
    (1-low, 4-high)
    Alternative To
    Blue Laser
    RB545  1(Unique position)
    RB613 2PE-CF594, PE-Dazzle™ 594, BB630-P2 or StarBright™ Blue 615
    RB670 2PE-Cy5, BB660-P2 or StarBright™ Blue 675
    RB705 2PerCP-Cy5.5, BB700 or StarBright™ Blue 700
    RB744 1BB755-P
    RB780 1PE-Cy7 or BB790-P
    Yellow-Green Laser
    RY586 1PE
    RY610 1PE-CF594 or PE-Dazzle™ 594
    RY703 2PE-Cy5.5 or StarBright™ Yellow 720
    RY743 2PE/Fire™ 744
    RY775 2PE-Cy7 or StarBright™ Yellow 800
    Note: PE and PE tandem dyes are excited by both the blue and yellow-green lasers. Be sure to check your instrument configuration

     

     

    RB545
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    BD Horizon RealBlue™ 545 (RB545) Reagents

    RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488 nm blue laser that offer:

     

    • Minimal cross-laser excitation off the 561 nm yellow-green laser
    • Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

     

    Download the BD Horizon RealBlue™ 545 Brochure>

     
    RB545 vials
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    RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

    Performance

    BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

    RB545 Normalised Emission Filter Parameters
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    RB545 Spread Plots
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    Applications

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    BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilisation systems

     

    BuffersResults
    BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
    CellBlox™ Blocking BufferCompatible
    BD Cytofix™ Fixation BufferStable at least 24 hours
    1% PFAStable at least 24 hours
    BD Cytofix/Cytoperm™ Fixation and Permeabilisation SolutionCompatible with antibody staining before and after fixation
    BD FACS™ Permeabilising Solution IICompatible with antibody staining before and after fixation
    BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
    EDTA and HeparinCompatible
    BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
     
    RB613
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    RB670
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    RB705
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    RB744
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    RB780
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    RY586
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    RY610
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    RY703
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    RY775
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    “The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

     

    “The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

     

    Dr. Florian Ebner
    Distinguished Lab Scientist
    Cancer Pharmacology and Disease Positioning
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    For Research Use Only. Not for use in diagnostic or therapeutic procedures. Refer to Manufacturer's instructions for use and related user manuals and technical sheets before using this product as described. Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
    BD-76302