The BD Biosciences Webinar Series is an ongoing, informative program in which senior scientists, professionals in the community, and clinicians present online seminars covering a broad range of topics.
Simplifying Stem Cell Characterization and Analysis with a Personal Flow Cytometer
Date: December 9, 2015
Time: 1 PM EST / 10 AM PST / 6 PM GMT / 7 PM CET
Mirko Corselli, PhD
R&D Supervisor, BD Biosciences
Heterogeneous stem cell populations need to be characterized and quantified to fully realize their utility in downstream applications. Stem cell populations can easily be phenotyped based on their cell surface marker expression as well as the expression of key intracellular pluripotency and lineage-specific markers. We will discuss how combining the BD Accuri™ C6 flow cytometer with BD Biosciences reagents provides a unique research solution to facilitate stem cell research. Specific applications discussed in this webinar will include:
- The use of known cell surface markers and cell surface marker screening to identify unique surface signatures for the isolation of stem cells and their derivatives
- Effective cell preparation and procedural considerations when performing intracellular flow cytometry
- Analysis of neural, cardiac, and endoderm differentiation of human embryonic stem cells (hESCs) using intracellular flow cytometry
- The use of BD kits and BD Accuri C6 software templates to streamline setup and analysis of experiments; including examples from characterizing mesenchymal stromal cells (MSC)
Mirko Corselli, is a senior stem cell scientist at BD Biosciences. Dr. Corselli obtained a PhD in Clinical and Experimental Hematology and Oncology from the University of Genova, Italy. He then transferred to the University of California, Los Angeles to continue his research as a postdoctoral fellow. Most of his academic work has focused on the biology and therapeutic potential of human mesenchymal stromal/stem cells (MSCs). He has developed multicolor flow cytometry-based strategies for the purification and characterization of MSCs from multiple human tissues. He is currently employed as an R&D supervisor at BD Biosciences, San Diego, CA, where he leads R&D application support projects and manages the cell function reagent portfolio.
Class 1 Laser Product
For Research Use Only. Not for use in diagnostic or therapeutic procedures.