The BD Biosciences Webinar Series is an ongoing, informative program in which senior scientists, professionals in the community, and clinicians present online seminars covering a broad range of topics.
10-Color Panel Design on the BD FACSCanto™ System
Date: Monday, September 26, 2016
Time: 12 PM EST / 9 AM PST / 5 PM GMT / 6 PM CET
BD Fellow, Principal Scientist, BD Life Sciences
Q2 Solutions (Quintiles/Quest), Scientific Advisor Flow Cytometry
Quality flow cytometry data starts with proper panel design. Fluorochrome choices, instrument configurations/setup and knowledge of the biology being studied are important factors when considering panel choices. In this webinar, we will provide you with a foundation to easily build the best possible panels to answer your experimental questions by using the 10‐color capability of the BD FACSCanto™ flow cytometer. We will follow up the foundational introduction with an overview of user defined panels for research use only, demonstrating how to maximize resolution of cell populations when running the BD FACSCanto system. This overview will include examples from testing that Quintiles* has done when developing its user defined tests, and the individual standard operating procedures used during this process.
Alan Stall is a BD Fellow for R&D at BD Biosciences. Alan has over 38 years of flow cytometry experience. He was an Assistant Professor in Microbiology and Director of the Comprehensive Cancer Center Flow Cytometry Core at Columbia University before joining BD, where he has spent the last 20 years. There he was a concept creator/R&D lead and co‐developer for BD™ CS&T beads used with the Cytometry Setup and Tracking system in BD FACSDiva™ software. He was also the Systems Engineering Lead for the BD™ FC Beads and BD FACSuite™ Automated Assay Setup system. His contributions at BD reveal his talent as a thought leader and his finesse at executing strategies to ensure that tools reach the marketplace and are supported by the education resources necessary for field implementation and use.
Mark Edinger is the Scientific Advisor for Flow Cytometry at Q2 Solutions. As the scientific advisor, Mark is responsible for flow cytometry assay design and implementation. He works with both the Translational Biomarkers Laboratory to develop assays and the Global Cytometry Group to produce assays, and is the architect of the systems and workflows used in Q2 Solutions’ flow cytometry assay development and production laboratories.
Mark began his career at the Cleveland Clinic Foundation, where he pioneered flow cytometry in the late 1970s, building both clinical and research core flow cytometry laboratories. During his 21 years at the Cleveland Clinic, Mark developed many of the techniques and assays employed today for clinical practice and academic research. In 1998, Mark joined BD, where he founded the Technical Applications Group, and later the R&D Systems Validation Laboratory. Mark joined Quintiles in 2012 and has been part of a collaboration which introduced the quantitative standardization of flow cytometers, along with other state‐of‐the‐art practices. Mark became part of the Q2 Solutions team when the company was officially launched in June 2015 as a result of a Quintiles and Quest Diagnostics joint venture.
*The information to be presented by Q2 Solutions represents Q2 Solutions’ views only. The content has not been reviewed and approved by BD Biosciences therefore not endorsed by BD Biosciences.