BD Horizon RealYellow™
and BD Horizon RealBlue™ Reagents

Still Using PerCP and PE Tandem Dyes?

Cleaner Alternatives Are Here.

The family of BD Horizon RealYellow^™ and BD Horizon RealBlue^™ Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

Driven by next-generation BD^® dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

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Still Using PerCP- and PE-Based Tandem Dyes? Time for an Upgrade.

Format	Spectral	Conventional	Spillover (1-low, 4-high)	Alternative To
Blue Laser
RB545	✓		1	(Unique position)
RB613	✓	✓	2	PE-CF594, PE-Dazzle™ 594, BB630-P2 or StarBright™ Blue 615
RB670	✓	✓	2	PE-Cy5, BB660-P2 or StarBright™ Blue 675
RB705	✓	✓	2	PerCP-Cy5.5, BB700 or StarBright™ Blue 700
RB744	✓	✓	1	BB755-P
RB780	✓	✓	1	PE-Cy7 or BB790-P
Yellow-Green Laser
RY586	✓	✓	1	PE
RY610	✓	✓	1	PE-CF594 or PE-Dazzle™ 594
RY655	✓	✓	3	PE-Cy5 or StarBright^™ Yellow 665
RY703	✓	✓	2	PE-Cy5.5 or StarBright™ Yellow 720
RY743	✓	✓	2	PE/Fire™ 744
RY775	✓	✓	2	PE-Cy7 or StarBright™ Yellow 800

Note: PE and PE tandem dyes are excited by both the blue and yellow-green lasers. RY655 signal may be reduced compared to PE-Cy5 on a conventional cytometer when using a filter centered at 670 nm due to the lower em-max wavelength. Be sure to check your instrument configuration

RB545

BD Horizon RealBlue™ 545 (RB545) Reagents

RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:

Minimal cross-laser excitation off the 561-nm yellow-green laser
Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

Download the BD Horizon RealBlue™ 545 Brochure>

RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

View available reagents >

Performance

BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

RB545 Normalized Emission Filter Parameters

Applications

1/4

BD Horizon™ RB545 Reagents support the detection of high-expression surface and intracellular markers

RB545 reagents can resolve high-expression surface and intracellular markers and are available in a wide array of specificities. RB545 reagents can be used with PE and FITC on spectral flow cytometers to meet your specific panel design needs.

View Available Reagents >

RB545 and FITC can be used together in spectral flow cytometry

BD Horizon RealBlue™ 545 Reagents are a superior alternative to FITC when used together with BD Horizon Brilliant™ Blue 515 (BB515) Reagents in high-parameter spectral flow cytometry panels

RB545 reagents can help improve resolution in high-parameter spectral flow cytometry panels.

Human PBMCs were stained with two sets of reagents, moderately similar CD4 RB545 and CD127 FITC, and highly similar CD4 FITC and CD127 BB515. Co-expressed markers were selected to demonstrate impact of spectral unmixing with different levels of similarity. Resolution was impacted when reagents were highly similar, shown in bottom set of CD4 FITC and CD127 BB515. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with FlowJo™ Software.

View available reagents >

BD Horizon RealBlue™ 545 Reagents offer stable reagent performance and photostability

RB545 reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.

Human whole blood was stained with human CD4 (SK3) RB545 reagent, followed by lysis with BD FACS™ Lysing Solution using four different batches of reagent (left). Mouse splenocytes were stained with mouse CD8a (53-6.7) using four different batches of reagent (right). All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.

View available reagents >

BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
CellBlox™ Blocking Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RB613

BD Horizon RealBlue™ 613 (RB613) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

RB613 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

A bright fluorochrome to support the detection of low-expression surface and intracellular markers
Significantly reduced cross-laser excitation off the 561-nm yellow-green laser
Compatibility with conventional and spectral flow cytometers with the appropriate detectors

Download the BD Horizon RealBlue^™613 and BD Horizon RealYellow ^™ 610>

RB613 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RB613 reagents increase panel design flexibility off the 488-nm blue laser, offering significantly reduced cross-laser excitation in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealBlue™ 613 Reagents are the best choice in this position for flow cytometers with a 488-nm blue laser

Normalized emission profile of RB613 and PE-CF594 fluorochromes.

RB613 shows reduced spillover spread into the corresponding yellow-green detectors.

Applications

1/3

BD Horizon™ RB613 Reagents support the detection of low-expression surface and intracellular markers

RB613 reagents can clearly resolve low-expression surface and intracellular markers and are available in a wide array of specificities.

BD Horizon™ RB613 Reagent resolves CD279 (PD-1) well

Intracellular transcription factor detected using BD Horizon™ RB613 FoxP3 Reagent

View Available Reagents >

BD Horizon™ RB613 Reagents have excellent resolution as demonstrated in a 22-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter.

BD Horizon RealYellow^™ and BD Horizon RealBlue^™ Reagents resolve well when run together in a spectral flow cytometry panel.

Gating strategy for detection of T cell subsets

View available reagents >

BD Horizon™ RB613 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB613 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealBlue^™ RB613 Regents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RB670

BD Horizon RealBlue™ 670 (RB670)
Reagents—A superior alternative to PE-Cy5

RB670 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

Significantly reduced cross-laser excitation off the 561-nm yellow-green laser
A bright fluorochrome to support the detection of low-expression surface and intracellular markers
Improved spillover compared to PE-Cy5, NovaFluor™ Blue 660 and StarBright™ Blue 675
Reduced monocyte background compared to PE-Cy5
Compatibility with conventional (with the appropriate detector) and spectral flow cytometers

RB670 reagents can be used in place of or along with PE-Cy5 on appropriately configured cytometers

Increase panel design flexibility with RB670 reagents, designed to reduce cross-laser excitation and available in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealBlue^™ 670 Reagents are a cleaner alternative to PE-Cy5, StarBright^™ Blue 675 and NovaFluor^™ Blue 660

Normalized emission profile of RB670, PE-Cy5, StarBright^™ Blue 675 and NovaFluor^™ Blue 660-120s fluorochromes acquired on a BD FACSymphony^™ A5 SE Cell Analyzer.

RB670 has minimal monocyte background.

Applications

1/3

BD Horizon™ RB670 Reagents support the detection of low-expression surface and intracellular markers

RB670 reagents can clearly resolve low-expression surface and intracellular markers and are available in a wide array of specificities.

BD Horizon™ RB670 Reagent resolves CD279 (PD-1) well

Intracellular transcription factor detected using BD Horizon™ RB670 FoxP3 Reagent

View available reagents >

BD Horizon™ RB670 Reagents have excellent resolution as demonstrated in a 18-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter.

BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents resolve well when run together in a spectral flow cytometry panel.

Gating strategy for detection of CD56+ NK cells and NK T-like cells

View available reagents >

BD Horizon™ RB670 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB670 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealBlue™ RB670 Regents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution 2	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RB705

BD Horizon RealBlue™ 705 (RB705) Reagents

The superior alternative to PerCP-Cy5.5 and BD Horizon Brilliant™ Blue 700 (BB700) Reagents

RB705 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

A bright fluorochrome to support the detection of low-expression surface and intracellular markers
Minimal cross-laser excitation off the 561-nm yellow-green laser
Reduced background compared to BB700 with no additional wash recommended

Download the BD Horizon RealBlue^™705 Brochure>

RB705 reagents can be used instead of PerCP-Cy5.5 or BB700 on conventional flow cytometers or with PerCP-Cy5.5 or BB700 on spectral flow cytometers

RB705 reagents increase panel design flexibility off the 488-nm blue laser, offering minimal cross-laser excitation in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealBlue™ 705 Reagents provide minimal cross-laser excitation off the 561-nm yellow-green laser

Normalized emission profile of RB705, BB700 and PerCP-Cy5.5 fluorochromes.

Spillover comparison of PerCP-Cy5.5 and BB700 with RB705

Compared to PerCP-Cy5.5 and BB700, RB705 shows lower spillover spread into the corresponding yellow-green, red, UV and violet detectors.

Applications

1/4

BD Horizon™ RB705 Reagents support the detection of low-expression surface and intracellular markers

RB705 reagents can clearly resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RB705 reagents can be used instead of PerCP-Cy5.5 or BB700 on conventional flow cytometers or with PerCP-Cy5.5 or BB700 on spectral flow cytometers to meet your specific panel design needs.

BD Horizon™ RB705 Reagent resolves CD279 well

BD Horizon™ RB705 IFNγ Reagent cytokine detection

View available reagents >

BD Horizon™ RB705 Reagent resolves well in a 12-color T cell inhibitory panel run on the BD FACSLyric™ Cell Analyzer.

RB705 is a bright fluorochrome that works well to resolve low-antigen expression surface markers such as TIM-3.

12-color T cell inhibitory panel gating strategy

Inhibitory marker expression in CD8+ T-cell subsets

RB705 resolves TIM-3 in activated T cells

View available reagents >

BD Horizon™ RB705 Reagents have excellent resolution as demonstrated in a 17-color spectral flow cytometry panel run on the BD FACSymphony™ A5 SE Cell Analyzer.

BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents resolve well when run together in a spectral flow cytometry panel.

17-color activated T-cell panel design strategy

Inhibitory receptor expression at different levels of CD8+ T cell activation

Intracellular cytokine expression at different levels of CD8+ T cell activation

View available reagents >

BD Horizon™ RB705 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB705 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.

RB705 lot-to-lot consistency across made-to-stock and BD OptiBuild On-Demand Reagents

View available reagents >

BD Horizon RealBlue™ 705 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
CellBlox™ Blocking Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RB744

BD Horizon RealBlue™ 744 (RB744) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

RB744 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488-nm blue laser to offer:

A bright fluorochrome to support the detection of low-expression surface and intracellular markers
Minimal cross-laser excitation off the 561-nm yellow-green laser
Compatibility with appropriately configured* BD FACSymphony™ Analyzers and spectral flow cytometers

*Filter centered near 750 nm, e.g., 750/30

Download the BD Horizon RealBlue™ 744 Reagent brochure >

RB744 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RB744 reagents increase panel design flexibility off the 488-nm blue laser, offering minimal cross-laser excitation in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealBlue™ 744 Reagents provide minimal cross-laser excitation off the 561-nm yellow-green laser

Normalized emission profile of RB744 fluorochromes

RB744 shows minimal spillover spread into the corresponding yellow-green, red, UV and violet detectors.

Applications

1/3

BD Horizon™ RB744 Reagents support the detection of low-expression surface and intracellular markers

RB744 reagents can clearly resolve low-expression surface and intracellular markers and are available in a wide array of specificities.

BD Horizon™ RB744 Reagent resolves CD25 well

View available reagents >

BD Horizon™ RB744 Reagents have excellent resolution as demonstrated in a 17-color spectral flow cytometry panel run on the BD FACSymphony™ A5 SE Cell Analyzer.

BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents resolve well when run together in a spectral flow cytometry panel.

View available reagents >

BD Horizon™ RB744 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB744 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealBlue™ 744 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
CellBlox™ Blocking Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RB780

BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7

RB780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser to offer:

Minimal cross-laser excitation off the 561-nm yellow-green laser
Bright fluorochrome to support the detection of low-expression surface and intracellular markers
Reduced monocyte background compared to PE-Cy7

Download the BD Horizon RealBlue™ 780 Reagents Brochure >

RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers

RB780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.

View available reagents >

Performance

BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7

Normalized emission profile of PE-Cy7 and RB780 with RB780 minimal excitation off the 561-nm yellow-green laser.

Human whole blood was stained with BD Horizon^™ RB780 Human CD4 (SK3) Reagent, acquired and compensated on a BD FACSymphony^™ A5 SE Cell Analyzer.

BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

Applications

1/3

BD Horizon™ RB780 Reagents support the detection of low-expression surface and intracellular markers

RB780 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers to meet your specific panel design needs.

View Available Reagents >

RB780 reagents and PE-Cy7 can be used together in spectral flow cytometry

BD Horizon™ RB780 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB780 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.

Human whole blood was stained with human CD4 (SK3) RB780, followed by lysis with BD FACS™ Lysing Solution using seven different batches of reagent (left). Mouse splenocytes were stained with mouse CD8a 53-6.7 using six different batches of reagent (right). All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.

View available reagents >

BD Horizon RealBlue™ 780 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
CellBlox™ Blocking Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RY586

Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents

BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:

Minimal cross-laser excitation off the 488-nm blue laser
Bright fluorescence to detect low-expression surface and intracellular markers

Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.

Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.

View Available Reagents >

Download BD Horizon RealYellow™ 586 Reagents Brochure >

Excellent Resolution

Generate reliable data that clearly resolve even the most challenging cell populations by leveraging bright, clean fluorochromes that minimize spillover and background.

Flexible Panel Design

Maximize panel design options for both conventional and spectral flow cytometry by using reagents that deliver minimal cross-laser excitation in hundreds of antibody specificities.

High-Dimensional Analysis

Reveal more insights from the same sample by expanding the utility of blue and yellow-green laser channels with reagents that enable the simultaneous use of more detectors.

Performance

1/5

Click through the slides below to view performance data.

Clean & Bright

BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation

BD Horizon RealYellow™ 586 Reagents exhibit minimal cross-laser excitation off the blue laser, resulting in significantly less spillover into blue detectors than PE. At the same time, they offer minimal background and high resolution comparable to PE.

View Available Reagents

Versatile Applications

BD Horizon RealYellow™ 586 Reagents have versatile applications

RY586 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RY586 reagents can be used instead of PE on conventional flow cytometers or with PE on spectral flow cytometers equipped with the 561-nm yellow-green laser to meet your specific panel design needs.

Intracellular cytokine marker RY586 data

View Available Reagents

Spectral Flow Cytometry

BD Horizon RealYellow™ Reagents can be used with spectral flow cytometry

RY586 can be used with PE on spectral flow cytometers. Since RY586 provides comparable brightness to PE, it is ideal for detecting low-expression markers. Using RY586 and PE in the same spectral flow cytometry panel enables high-dimensional analysis of more low-expression surface and intracellular markers.

Stable & Consistent

Performance stability of BD Horizon RealYellow™ Reagents RY586 offers stable reagent performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents as well as proven photostability when exposed to typical lab lighting or dimmed core lab lighting.

RY586 made-to-stock reagents and BD OptiBuild™ On-Demand Reagents show consistent performance between each other and across multiple lots.

Human whole blood was stained with human CD4 (SK3) RY586, followed by lysis with BD FACS™ Lysing Solution. Mouse splenocytes were stained with mouse CD8a 53-6.7. All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.

View Available Reagents

BD Horizon RealYellow™ Reagents offer wide compatibility

BD Horizon RealYellow™ Reagents are compatible with a variety of common fixation and permeabilization systems, including:

BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit
BD Pharmingen™ Transcription Factor Buffer Set
BD FACS™ Lysing Solution
BD Pharm Lyse™ Lysing Buffer
BD Cytofix™ Fixation Buffer
BD FACS™ Permeabilizing Solution 2
BD Phosflow™ Perm III
BD Horizon™ Brilliant Stain Buffer (BSB)
1% PFA
EDTA and heparin
CellBlox™ Blocking Buffer

View Available Reagents

RY610

BD Horizon RealYellow™ 610 (RY610) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

RY610 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 561-nm yellow-green laser to offer:

A bright fluorochrome to support the detection of markers across a variety of expression levels
A clean alternative to BD Horizon™ PE-CF594 and PE-Dazzle™ 594 Reagents with minimal cross-laser excitation off the 488-nm blue laser
Compatibility with conventional and spectral flow cytometers with the appropriate detectors

Download the BD Horizon RealBlue™ 613 and BD Horizon RealYellow ™ 610>

RY610 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RY610 reagents, available in hundreds of antibody specificities, provide improved spillover compared to PE-CF594 on instruments with a 561-nm yellow-green laser for additional panel design flexibility in high parameter and spectral flow cytometry.

View available reagents >

Performance

BD Horizon RealYellow™ 610 Reagents have a clean emission profile with minimal spillover for flow cytometers with a 561-nm yellow-green laser

Normalized emission profile of RY610 and PE-CF594 fluorochromes.

RY610 shows reduced spillover spread into the corresponding blue detectors.

Applications

1/3

BD Horizon™ RY610 Reagents support the detection of surface and intracellular markers across a range expression levels

RY610 reagents demonstrate excellent performance for surface and intracellular markers at a variety of antigen densities and are available in a wide array of specificities.

BD Horizon™ RY610 Reagent resolves Ly-6C well

Intracellular cytokine detected using BD Horizon™ RY610 IFN-γ Reagent

View Available Reagents >

BD Horizon™ RY610 Reagents have excellent resolution as demonstrated in a 22-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter.

BD Horizon RealYellow^™ and BD Horizon RealBlue^™ Reagents resolve well when run together in a spectral flow cytometry panel.

View available reagents >

BD Horizon™ RY610 Reagents offer stable reagent performance and photostability

The BD Horizon^™ RY610 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild^™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealYellow^™610 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RY655

BD Horizon RealYellow™ 655 (RY655) Reagents

A bright new option for high parameter and spectral flow cytometry panels

RY655 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 561-nm yellow-green laser to offer:

A bright fluorochrome to support the detection of low expression surface and intracellular markers
Limited cross-laser excitation from the 488-nm blue laser
Reduced nonspecific monocyte binding compared to legacy PE tandem fluorochromes
Compatible with appropriately configured* conventional and spectral flow cytometers

*Filter centered near 660 nm e.g., 660/30 or 670/30. Note that a 670/30 configuration on a conventional instrument may not capture the max signal and thus higher antigen density markers should be assigned.

RY655 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RY655 reagents increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealYellow™ 655 Reagents are very bright with reduced spillover and monocyte background

Normalized emission profile of CD4 (SK3) RY655 PE-Cy5, NovaFluor^™ Yellow 660 (NFY660) and PE/Fire™ 640, and CD4 (RPA-T4) StarBright^™ Yellow 665 (SBY665).

RY655 Reduced Spread into RB670 Compared to PE-Cy5

RY655 shows reduced spillover spread into RB670.

RY655 provides reduced nonspecific binding.

Applications

1/4

BD Horizon™ RY655 Reagents support the detection of low-expression surface and intracellular markers

RY655 reagents demonstrate excellent performance for surface and intracellular markers with low antigen densities and are available in a wide array of specificities.

View Available Reagents >

RY655 works well with other fluorochromes

RY655 can be used with RB670 on instruments with both blue and yellow-green lasers and the appropriate detectors.

View available reagents >

BD Horizon™ RY655 Reagents have excellent resolution as demonstrated in a 30-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter

BD Horizon RealYellow^™and BD Horizon RealBlue^™ Reagents resolve well when run together in a spectral flow cytometry panel.

Fluorochrome marker assignment for a 30-color spectral flow cytometry panel

BD Horizon RealYellow and RealBlue Reagents in a 30-color Human Immunophenotyping Panel

View available reagents >

BD Horizon™ RY655 Reagents offer stable reagent performance and photostability

The BD Horizon™ RY655 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealYellow^™655 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution 2	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RY703

BD Horizon RealYellow™ 703 (RY703) Reagents

A superior alternative to PE-Cy5.5 for high-parameter and spectral flow cytometry panels

RY703 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 561-nm yellow-green laser to offer:

A bright fluorochrome to support the detection of low expression surface and intracellular markers
A cleaner alternative to PE-Cy5.5 and StarBright™ Yellow 720 (SBY720) Reagent with reduced cross-laser excitation
Reduced nonspecific monocyte binding
Compatibility with conventional and spectral flow cytometers with the appropriate detectors

Download the BD Horizon RealYellow™ 703 Reagent Brochure >

RY703 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RY703 reagents provide improved spillover compared to PE-Cy5.5 on instruments with a 561-nm yellow-green laser for additional panel design flexibility in high-parameter and spectral flow cytometry.

View available reagents >

Performance

BD Horizon RealYellow™ 703 Reagents are very bright with reduced spillover and monocyte background

Normalized emission profile of RY703, PE-Cy5.5 and StarBright™ Yellow 720 (SBY720) Reagent.

RY703 shows reduced spillover spread into the corresponding blue detectors.

RY703 has minimal monocyte background

Applications

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BD Horizon™ RY703 Reagents support the detection of low-expression intracellular and surface markers

RY703 reagents demonstrate excellent performance for surface and intracellular markers at low antigen densities and are available in a wide array of specificities.

RY703-Reagent-Lower-Expression-Intracellular-Markers

RY703-Low-Antigen-Density-Surface-Markers

View Available Reagents >

BD Horizon™ RY703 Reagents have excellent resolution as demonstrated in an 18-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter

BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents resolve well when run together in a spectral flow cytometry panel.

RY703-18-Color-Spectral-Flow-Cytometry-Panel

RY703-and-RB705-Human-Immunophenotyping-Panel

View available reagents >

BD Horizon™ RY703 Reagents offer stable reagent performance and photostability

BD Horizon^™ RY703 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild^™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealYellow^™703 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RY743

BD Horizon RealYellow™ 743 (RY743) Reagents

A bright new option for high parameter and spectral flow cytometry panels

RY743 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 561-nm yellow-green laser to offer:

A bright fluorochrome to support the detection of low expression surface and intracellular markers
Limited cross-laser excitation from the 488-nm blue laser
Reduced nonspecific monocyte binding compared to legacy PE tandem fluorochromes
Compatibility with appropriately configured* BD FACSymphony™ Analyzers and spectral flow cytometers

*Filter centered near 750 nm, e.g., 750/30

RY743 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RY743 reagents increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.

View available reagents >

Performance

BD Horizon RealYellow™ 743 Reagents are very bright with reduced spillover and monocyte background

Normalized emission profile of CD4 (SK3) RY743, PE/Fire™ 744 and NovaFluor™ Yellow 755 (NFY755).

RY743 shows reduced spillover spread into RB744.

RY743 provides reduced nonspecific binding.

Applications

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BD Horizon™ RY743 Reagents support the detection of low-expression surface and intracellular markers

RY743 reagents demonstrate excellent performance for surface and intracellular markers at low antigen densities and are available in a wide array of specificities.

RY743-Reagent-Lower-Expression-Surface-Markers

View Available Reagents >

RY743 works well with other fluorochromes

Cleaner Fluorochrome for High Parameter Flow Cytometry

RY743 can be used with RB744 on instruments with both blue and yellow-green lasers and the appropriate detectors

View available reagents >

BD Horizon™ RY743 Reagents have excellent resolution as demonstrated in an 18-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter

BD Horizon RealYellow^™ and BD Horizon RealBlue^™ Reagents resolve well when run together in a spectral flow cytometry panel.

18-Color Spectral Flow Cytometry Panel Fluorochrome Marker Assignment

Helios RY743 co-stained with CD62L BV480 on a BD FACSDiscover™ S8 Cell Sorter

View available reagents >

BD Horizon™ RY743 Reagents offer stable reagent performance and photostability

The BD Horizon^™ RY743 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild^™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealYellow^™743 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

RY775

BD Horizon RealYellow™ 775 (RY775) Reagents

Superior alternative to PE-Cy7 for high-parameter and spectral flow cytometry panels

RY775 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 561-nm yellow-green laser to offer:

A bright fluorochrome to support the detection of low expression surface and intracellular markers
A cleaner alternative to PE-Cy7 and StarBright™ Yellow 800 (SBY800) with reduced cross-laser excitation
Reduced nonspecific monocyte binding compared to PE-Cy7
Compatibility with conventional and spectral flow cytometers with the appropriate detectors

Download the BD Horizon RealYellow™ 775 Reagent brochure >

RY775 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

RY775 reagents provide improved spillover compared to PE-Cy7 on instruments with a 561-nm yellow-green laser for additional panel design flexibility in high-parameter and spectral flow cytometry.

View available reagents >

Performance

BD Horizon RealYellow™ 775 Reagents are very bright with reduced spillover and monocyte background

Normalized emission profile of RY775, PE-Cy7 and StarBright™ Yellow 800

RY775 shows reduced spillover spread into the corresponding blue detectors.

RY775 provides improved nonspecific binding.

Applications

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BD Horizon™ RY775 Reagents support the detection of low-expression surface and intracellular markers

RY775 reagents demonstrate excellent performance for surface and intracellular markers at low antigen densities and are available in a wide array of specificities.

RY775-Reagent-Lower-Expression-Surface-Markers

View Available Reagents >

BD Horizon™ RY775 Reagents have excellent resolution as demonstrated in an 18-color spectral flow cytometry panel run on the BD FACSDiscover™ S8 Cell Sorter

BD Horizon RealYellow^™ and BD Horizon RealBlue^™ Reagents resolve well when run together in a spectral flow cytometry panel.

18-Color-Flow-Cytometry-Panel-Fluorochrom-Marker-Assignment

RY775-and-RB780-reagent-Spectral-Human-Immunophenotyping-Panel

View available reagents >

BD Horizon™ RY775 Reagents offer stable reagent performance and photostability

The BD Horizon^™ RY775 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild^™ On-Demand Reagents. They also offer photostability when exposed to typical lab lighting or dimmed core lab lighting.

View available reagents >

BD Horizon RealYellow^™775 Reagents are compatible with a broad range of fixation and permeabilization systems

Buffers	Results
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffer	Compatible
BD Cytofix™ Fixation Buffer	Stable at least 24 hours
1% PFA	Stable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	Compatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution II	Compatible with antibody staining before and after fixation
BD Phosflow™ Perm Buffer III	Compatible with antibody staining before and after fixation
EDTA and Heparin	Compatible
BD Horizon™ Brilliant Stain Buffer (BSB)	Compatible

"BD Horizon RealYellow™ 586 (RY586) Reagents look like the best alternative for PE and will simplify panel design on conventional flow cytometers. They can also be an additional dye for spectral panel design.”

Gert Van Isterdael, PhD

VIB Ghent University, Belgium

“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

Dr. Florian Ebner

Distinguished Lab Scientist

Cancer Pharmacology and Disease Positioning

“I’m really excited about the BD Horizon RealBlue^™ 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi

Researcher

Bernhard Nocht Institute for Tropical Medicine

Gert Van Isterdael, PhD

VIB Ghent University, Belgium

Dr. Florian Ebner

Distinguished Lab Scientist

Cancer Pharmacology and Disease Positioning

“I’m really excited about the BD Horizon RealBlue^™ 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi

Researcher

Bernhard Nocht Institute for Tropical Medicine

Gert Van Isterdael, PhD

VIB Ghent University, Belgium

RESOURCES

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Brochures

BD Horizon RealYellow™ and RealBlue™ Reagents Brochure

BD Horizon RealBlue™ 545 Reagents

BD Horizon RealBlue™ 613 and BD Horizon RealYellow ™ 610

BD Horizon RealBlue™ 705 Reagents

BD Horizon RealBlue™ 744 Reagents

BD Horizon RealBlue™ 780 Reagents

BD Horizon RealYellow™ 586 Reagents

BD Horizon RealYellow™ 775 Reagents

BD Horizon RealYellow™ 703 Reagents

Fluorochrome Faceoff Brochure

Performance Guide

Fluorochrome Performance Guide

Fluorochrome Performance Chart

BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. CellBlox is a trademark of Thermo Fisher Scientific.

BD Horizon RealYellow™and BD Horizon RealBlue™ Reagents

Still Using PerCP- and PE-Based Tandem Dyes? Time for an Upgrade.

Note: PE and PE tandem dyes are excited by both the blue and yellow-green lasers. RY655 signal may be reduced compared to PE-Cy5 on a conventional cytometer when using a filter centered at 670 nm due to the lower em-max wavelength. Be sure to check your instrument configuration

BD Horizon RealBlue™ 545 (RB545) Reagents

RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 613 (RB613) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

RB613 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealBlue™ 613 Reagents are the best choice in this position for flow cytometers with a 488-nm blue laser

BD Horizon RealBlue™ RB613 Regents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 670 (RB670) Reagents—A superior alternative to PE-Cy5

RB670 reagents can be used in place of or along with PE-Cy5 on appropriately configured cytometers

BD Horizon RealBlue™ 670 Reagents are a cleaner alternative to PE-Cy5, StarBright™ Blue 675 and NovaFluor™ Blue 660

BD Horizon RealBlue™ RB670 Regents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 705 (RB705) Reagents

The superior alternative to PerCP-Cy5.5 and BD Horizon Brilliant™ Blue 700 (BB700) Reagents

RB705 reagents can be used instead of PerCP-Cy5.5 or BB700 on conventional flow cytometers or with PerCP-Cy5.5 or BB700 on spectral flow cytometers

BD Horizon RealBlue™ 705 Reagents provide minimal cross-laser excitation off the 561-nm yellow-green laser

BD Horizon RealBlue™ 705 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 744 (RB744) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

*Filter centered near 750 nm, e.g., 750/30

Download the BD Horizon RealBlue™ 744 Reagent brochure >

RB744 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealBlue™ 744 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7

RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers

BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7

BD Horizon RealBlue™ 780 Reagents are compatible with a broad range of fixation and permeabilization systems

Introducing BD Horizon RealYellow™ 586 (RY586) Reagents

Clean & Bright

BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation

BD Horizon RealYellow™ 610 (RY610) Reagents

A bright new option for high-parameter and spectral flow cytometry panels

RY610 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealYellow™ 610 Reagents have a clean emission profile with minimal spillover for flow cytometers with a 561-nm yellow-green laser

BD Horizon RealYellow™ 610 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow™ 655 (RY655) Reagents

A bright new option for high parameter and spectral flow cytometry panels

*Filter centered near 660 nm e.g., 660/30 or 670/30. Note that a 670/30 configuration on a conventional instrument may not capture the max signal and thus higher antigen density markers should be assigned.

RY655 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealYellow™ 655 Reagents are very bright with reduced spillover and monocyte background

BD Horizon RealYellow™ 655 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow™ 703 (RY703) Reagents

A superior alternative to PE-Cy5.5 for high-parameter and spectral flow cytometry panels

RY703 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealYellow™ 703 Reagents are very bright with reduced spillover and monocyte background

BD Horizon RealYellow™ 703 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow™ 743 (RY743) Reagents

A bright new option for high parameter and spectral flow cytometry panels

*Filter centered near 750 nm, e.g., 750/30

RY743 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealYellow™ 743 Reagents are very bright with reduced spillover and monocyte background

BD Horizon RealYellow™ 743 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow™ 775 (RY775) Reagents

Superior alternative to PE-Cy7 for high-parameter and spectral flow cytometry panels

RY775 reagents can be used in higher parameter flow cytometry panels to increase biological resolution

BD Horizon RealYellow™ 775 Reagents are very bright with reduced spillover and monocyte background

BD Horizon RealYellow™ 775 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow™
and BD Horizon RealBlue™ Reagents

BD Horizon RealBlue^™ RB613 Regents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealBlue™ 670 (RB670)
Reagents—A superior alternative to PE-Cy5

BD Horizon RealBlue^™ 670 Reagents are a cleaner alternative to PE-Cy5, StarBright^™ Blue 675 and NovaFluor^™ Blue 660

Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents

BD Horizon RealYellow^™610 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow^™655 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow^™703 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow^™743 Reagents are compatible with a broad range of fixation and permeabilization systems

BD Horizon RealYellow^™775 Reagents are compatible with a broad range of fixation and permeabilization systems