BD Accuri C6
NOTE: This update does not impact the BD Accuri™ C6 Plus
BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. All current service contracts will be honored. Current extended warranties will not be affected and will be supported until they expire. One-year and multi-year extended warranty renewals will continue to be offered until April 15, 2020, provided these contracts end on or before April 15, 2021. After contracts terminate, BD will support the BD Accuri C6 system for as long as reasonably possible, depending on parts availability and other matters.
For information and special offers on the new BD Accuri C6 Plus personal flow cytometer, please visit our page www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd-accuri/m/1294932/overview.
Frequently Asked Questions
Q: Will BD Biosciences continue to sell BD Accuri C6 spare parts and consumables?
A: After April 15, 2021, our sale of BD Accuri C6 spare parts and consumables will be dictated by parts availability and other restrictions.
Q: Will BD Biosciences continue to offer BD Accuri C6 operator training?
A: BD Accuri C6 training and applications support at customer sites will be offered based on the availability of staff with product knowledge. Please contact your regional customer-training center for more information. In the US, contact: traininginfo@bd.com or call 1.877.232.8995, prompt 5.
Q: How will we obtain service after April 15, 2021?
A: After April 15, 2021, BD Accuri C6 instruments may obtain service from BD Biosciences depending on parts and personnel availability.
BD Accuri C6 Resources
Brochures
Catalogs
- BD Accuri™ Multicolor Reagents Catalog
- BD Multicolor Kit Catalog
- Kits and Templates for the BD Accuri™ C6
Experiment Sheets
- BD Accuri™ C6 and BD Pharmingen™ Apoptosis, DNA Damage and Cell Proliferation Kit:
- BD Accuri™ C6 and BD Pharmingen™ Stem Cell Marker Antibodies
- BD Accuri™ C6 and BD Stemflow™ hMSC Analysis Kit
- BD Accuri™ C6 and BD Stemflow™ Human Definitive and Pancreatic Endoderm Analysis Kit
- BD Accuri™ C6 and BD Stemflow™ Pluripotent Stem Cell Kits
Product Information Sheets
- Analysis of Cytokine Expression in Cancer Cell Lines Using Flow Cytometry
- BD Accuri Calcein AM Datasheet
- BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template
- BD Apoptosis Kits and Templates
- BD Cell Cycle and DNA Kits and Templates
- BD™ Cell Viability Kit
- BD Cycletest™ Plus DNA Reagent Kit
- BD™ Cytometric Bead Array (CBA) Reagents: A complete solution with the BD Accuri™ C6 Flow Cytometer
- BD Horizon™ Fixable Viability Stain Reagents
- BD Human Mesenchymal Stromal Cell Kit and Template
- BD Induced Pluripotent Stem Cell Kit and Template
- BD Intracellular T-Cell Kits and Templates
- BD Mouse B-Lymphocyte Kits and Templates
- BD Mouse T-Cell Cytokine and Treg Kits and Templates
- BD Mouse T-Lymphocyte Kits and Templates
- BD Naïve/Memory T- Cell Kits and Templates
- BD Pharmingen™ BrdU Flow Kits
- BD Pharmingen™ MitoStatus
- BD Pluripotent Stem Cell Kits and Templates
- BD Regulatory T-Cell Kits and Templates
- Combining Cell Migration and Immunophenotype Assessment Using Flow Cytometry
- Detecting Red Fluorescent Proteins on the BD Accuri™ C6
- Dose–Response Studies Using Flow Cytometry
- Immunophenotyping Cancer Cells Using Flow Cytometry
- Protein Phosphorylation Analysis Using Flow Cytometry
- Selectable Lasers Module for the BD Accuri™ C6 Flow Cytometer
- Special Order BD Accuri™ C6 Research Systems
- SPHERO™ Rainbow Calibration Particles
Product List
Quick Start Guides
- BD Accuri™ C6 Software Quick Start Guide - Analyze
- BD Accuri™ C6 Software Quick Start Guide - Batch Analysis
- BD Accuri™ C6 Software Quick Start Guide - BD CSampler™ Accessory
- BD Accuri™ C6 Software Quick Start Guide - Collect
- BD Accuri™ C6 Software Quick Start Guide - Statistics
Technical Documents
- Assessing Water Quality
- A Guide to Absolute Counting
- Analysis of 2102Ep Cells as a Pluripotent Stem Cell Reference Standard
- A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples
- Automating the Personal Flow Cytometer
- Baculovirus Titration
- Bioprocess Monitoring
- Continuous Measurement of Intracellular Calcium
- Determining Cell Concentration by Direct Volume
- Identification of Human Peripheral Blood Cell Populations
- Guide for Using BD™ Cytometric Bead Array (CBA) Kits
- Guide for Using BD™ Cytometric Bead Array (CBA) Flex Sets
- Guidelines for Journal Authors Using the BD Accuri™ C6
- Making Flow Cytometry Personal
- Multiple Methods for Detecting Apoptosis
- Multiparametric Analysis of Aquatic Organisms Using Flow Cytometry
- Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry
- Optical Filter Guidelines for Fluorescent Protein Analysis
- Platelet Counting with the BD Accuri™ C6 Flow Cytometer
- Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants
- Simplifying Complex Flow Cytometric Analyses
- Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy
- Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education
- Studying Cell Signaling with the BD Accuri™ C6 Flow Cytometer
- Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors: Thinking Outside the Orthogonal Box
- The Essential Cell Analysis Tool
- The Personal Flow Cytometer in the Flow Cytometry Core Facility
- Threshold and Analysis of Small Particles
Technical Specifications
Tools
- BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®)
- BD Accuri™ C6 Flow Cytometer Optical Filter Guide
User's Guides and Manuals
- BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher
- BD Accuri™ C6 Software User Guide
- BD CSampler™ Accessory Installation Guide
- BD CSampler™ Software User Guide
- Display of BD Accuri™ C6 Software-Generated FCS 3.0 Files Using FlowJo™ Version 8.8.7 or 9.4.11 for the Mac®
- Guide to the FCS Express™ BD Accuri™ Software Importer
Videos
- Initial setup procedure
- General preventative maintenance procedures
- SIP maintenance procedure
- CSampler alignment procedure
Webinars
- Analyzing Data Collected on the BD Accuri™ C6 Using FlowJo™ PDF | Video
- Cell and Platelet Counting Using the BD Accuri™ C6 Flow Cytometer PDF
- Cell Biology Applications of Real-Time Flow Cytometry PDF | Video
- Enabling a Deeper Understanding of What is on, in, and Made by the Cell PDF | Video
- Flow Cytometry Applications in Cell Biology and Cancer Research PDF | Video
- Flow Cytometry as a Tool for Microbial Analysis PDF | Video
- Integrating Flow Cytometry into Education PDF | Video
Templates
Download the catalog of available kits and templates, or select from the following table.
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Cancer and Cell Biology | BD Apoptosis Kits and Templates | BD Pharmingen™ | Annexin V FITC Apoptosis Detection Kit II | 556570 | Download |
| BD Pharmingen™ | Annexin V PE Apoptosis Detection Kit I | 559763 | Download | ||
| BD™ | MitoScreen (JC-1) Kit | 551302 | Download | ||
| BD Pharmingen™ | Caspase-3 PE Assay Kit | 550914 | Download | ||
| BD Pharmingen™ | Caspase-3 FITC Assay Kit | 550480 | Download | ||
| BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template | BD Pharmingen™ | Apoptosis, DNA Damage and Cell Proliferation Kit | 562253 | Download | |
| BD Cell Cycle and DNA Kits and Templates | |||||
| BD Cycletest™ Plus | DNA Reagent Kit | 340242 | Download | ||
| BD Pharmingen™ | FITC BrdU Flow Kit | 559619 | Download | ||
| BD Pharmingen™ | APC BrdU Flow Kit | 552598 | Download |
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Immunology | BD Naïve/Memory T-Cell Kits and Templates | BD Multitest™ | CD45RA/CD45RO/CD3/CD4 | 340571 | Download |
| BD Multitest™ | CD45RA/CD62L/CD3/CD4 | 340977 | Download | ||
| BD Pharmingen™ | Human Naïve/Memory T Cell Panel | 561438 | Download | ||
| BD Intracellular T-Cell Kits and Templates | |||||
| BD Pharmingen™ | Th1/Th2/Th17 Phenotyping Kit | 560751 | Download | ||
| BD FastImmune™ | IFN-γ/CD69/CD8/CD3 Kit | 346048 | Download | ||
| BD FastImmune™ | IFN-γ/IL-4 Kit | 340456 | Download | ||
| BD Regulatory T-Cell Kits and Templates | |||||
| BD Pharmingen™ | Human Regulatory T Cell Cocktail | 560249 | Download | ||
| BD Pharmingen™ | Human Th17/Treg Phenotyping Kit | 560762 | Download | ||
| BD Pharmingen™ | FoxP3 Staining Kit | 560132 | Download | ||
| BD Mouse B-Lymphocyte Kits and Templates | |||||
| BD Pharmingen™ | Mouse B-Lymphocyte Subset Antibody Cocktail | 558332 | Download | ||
| BD Pharmingen™ | Mouse B-Lymphocyte Activation Antibody Cocktail | 558063 | Download | ||
| BD Mouse T-Cell Cytokine and Treg Kits and Templates | |||||
| BD Pharmingen™ | Mouse Th1/Th2/Th17 Phenotyping Kit | 560758 | Download | ||
| BD Pharmingen™ | Mouse Th17/Treg Phenotyping Kit | 560767 | Download | ||
| BD Mouse T-Lymphocyte Kits and Templates | |||||
| BD Pharmingen™ | Mouse T-Lymphocyte Subset Antibody Cocktail | 558431 | Download | ||
| BD Pharmingen™ | Mouse T-Lymphocyte Activation Antibody Cocktail | 557908 | Download |
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Stem Cells | BD Pluripotent Stem Cell Kits and Templates | BD Stemflow™ | Human Pluripotent Stem Cell Sorting and Analysis Kit | 560461 | Download |
| BD Stemflow™ | Human Pluripotent Stem Cell Transcription Factor Analysis Kit | 560589 | Download | ||
| BD Stemflow™ | Human and Mouse Pluripotent Stem Cell Analysis Kit | 560477 | Download | ||
| BD Induced Pluripotent Stem Cell Kit and Template | BD Stemflow™ | Human iPSC Sorting and Analysis Kit | 562626 | Download | |
| BD Human Mesenchymal Stromal Cell Kit and Template | BD Stemflow™ | Human MSC Analysis Kit | 562245 | Download |
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Bead-Based Assays | BD™ Cytometric Bead Array (CBA) Reagents | BD™ | CBA Kits | See Kits | Download |
| BD™ | CBA Flex Sets | See Flex Sets | Download |
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Microbiology | BD Accuri™ C6 Eawag Water Quality Template | BD Accuri™ | Eawag Water Quality Template | — | Download |
| Category | Product Information Sheet | Brand | Kit | Cat. No. | Template |
|---|---|---|---|---|---|
| Instrument Setup | BD Accuri™ C6 Software User Guide | BD Accuri™ | 8 and 6 Peak Bead Template | 653144
653145 653160 |
Download |
| BD Accuri™ | 8 and 6 Peak Bead Template 24-tube Rack | 653144
653145 653160 |
Download |
Once you have downloaded a template, select File > Open Workspace or Template and open it. Gate positions, zoom level, thresholds, and compensation, run, and fluidics settings are all fully adjustable and may require optimization for different sample types. Once the settings are optimized for your experiment, click Run to begin data acquisition.
Note: The linked templates on this page are provided for the convenience of our research customers. Although they have not been validated, they have been used by BD or our customers to generate the sample data in the product information sheets and on the Sample Data tab. The templates have been designed using the current BD Accuri C6 software, version 264.21. They are not compatible with version 172.9, 227.4, or 202.8 software. Further optimization may be required before use, and it is the end user's responsibility to ensure that use of the template is appropriate to his or her research.
Class 1 Laser Product.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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