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Rat P450 Immunoblotting Antibodies

The following is a summary of the immunoblotting crossreactivities of antibodies which have applications to the study of rat cytochrome P450 enzymes in rat tissues. Antibodies manufactured by Daiichi were prepared in goats against purified rat cytochrome P450. Antibodies manufactured by BD Gentest™ are antipeptide antisera developed in rabbits. All antibodies are polyclonal.

FAQs (Frequently Asked Questions) about antibody products.

Specificty of Immunoblotting

  Purified Rat Cytochrome P450 Form (50 ng)  
Antiserum

1A1

1A2

1B1

2A1

2A2

2B1

2B2

2C6

2C11

2C13

2D1

2E1

3A2

4A1

Catalog Number Manufacturer

Anti-CYP1B1*
-
-
+++
-
-
-
-
 -
-
-
-
-
-
 -
458511 (Old A511) BD Gentest™
Anti-CYP3A2
-
-
 -
-
-
+
-
+
+
+
-
-
 +++
458223 Daiichi

*Specificity was determined by Western blotting a cDNA-expressed rat cytochrome P450 panel.

*:

Monoclonal Antibody
+++:
Very Strongly Crossreactive
++:
Strongly Crossreactive
+:
Crossreactive
(+):
Weakly Crossreactive
-:
Crossreactivity Not Detected
lm:
Lower Mobility
hm:
Higher Mobility
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