BD™ MALDI Sample Concentrator

A simple solution for concentrating and purifying multiple samples for MALDI mass spectrometer analysis.

BD™ MALDI Sample Concentrator is a silicone elastomer in the format of a 96-well or 100-well plate that fits on top of a typical MALDI plate. The product has holes in the silicone that form wells when placed and sealed on a MALDI plate. Liquid-tight sealing occurs naturally between the silicone and plate by electrostatic attraction. The product is used to concentrate liquid samples to dryness on the MALDI plate or to desalt and concentrate peptide and protein mixtures prior to MALDI analysis.

Features for 96-well Format

• Standard 96-well microplate footprint ensures automation compatibility
  
• Compatible with microplate-size MALDI plates found on a wide range of mass spectrometers
  
• Allows the concentration and desalting of up to 150µL of sample using common laboratory centrifuges (with microplate carriers)
  
• The concentration effect typically increases the sensitivity of the analysis by an order of magnitude or more
  
• Resulting spot size is about 1mm

Features for 100-well Format

• Compatible with 56mm x 56mm microplate-size MALDI plates used with Applied Biosystems Voyager™ MALDI mass spectrometers
  
• Allows the concentration and desalting of up to 50µL of sample using common laboratory vacuum concentrators
  
• The concentration effect typically increases the sensitivity of the analysis by an order of magnitude or more
  
• Resulting spot size is about 1mm
  

Figure 1: How to use the BD Maldi Concentrator.

Figure 2: Technical Examples and Data: MALDI MS of the trypsin digest products of bovine serum albumin (BSA).
	A high concentration (1000 nM) sample was spotted directly onto the MALDI target.
	A 10 nM sample was desalted, concentrated and spotted using the BD™ MALDI Sample Concentrator.
	The same 10 nM sample was desalted, concentrated and spotted using C18 resin-packed pipette tips.

MALDI MS of a mixture of 5 standard peptides
(a) BD™ MALDI Sample Concentrator	(b) Pipette tip with C18 resin
Figure 3: MALDI MS of a mixture of 5 standard peptides (angiotensin II, synthetic peptide P14R, ACTH fragment 18-39, bovine insulin oxidized B chain, and bovine insulin). (a) The samples were desalted, concentrated and spotted using the BD™ MALDI Sample Concentrator. (b) The same samples were desalted, concentrated and spotted using C18 resin-packed pipette tips. Peaks from 5 peptides were indicated with *.

Technical Documents

Guidelines for Use

• BD™ MALDI Sample Concentrator: 96-well Format -- [ Guidelines for Use ]
• BD™ MALDI Sample Concentrator: 100-well Format and BD™ MALDI Sample Concentrator Holder Kit -- [ Guidelines for Use ]

Research Papers

• Chen, X.; Murawski, A.; Kuang, G.; Sexton, D. J.; Galbraith, W. Sample Preparation for MALDI Mass Spectrometry Using an Elastomeric Device Reversibly Sealed on the MALDI Target. Analytical Chemistry, ASAP Article, Web Release Date: August 4, 2006 -- [ Abstract, Request electronic reprint from Analytical Chemistry (first 50 request free) ]
• Snovida, S. I.; Chen, V. C.; Krokhin, O.; Perreault, H. Isolation and Identification of Sialylated Glycopeptides from Bovine α₁-Acid Glycoprotein by Off-Line Capillary Electrophoresis MALDI-TOF Mass Spectrometry. Analytical Chemistry, ASAP Article, Web Release Date: July 27, 2006 -- [ Abstract (BD™ MALDI Sample Concentrator is used for on-target CE fraction collection) ]

Posters

• Trypsin Digestion of Proteins Immobilized on the Hydrophobic Surface of an Elastomeric Device --[ Poster presented at 2006 ASMS Annual Conference ]
• Improving Hydrophobic Peptide Recovery in MALDI MS using a Novel Desalting and Concentrating Method -- [ Poster presented at 2005 ASMS Annual Conference ]
• BD™ MALDI Sample Concentrator Products: A New Sample Preparation Technology for Improving Sensitivity and Throughput in MALDI Mass Spec -- [ Poster presented at 2005 ASMS Annual Conference ]
• Direct Protein 2D-LC MALDI with On-Target Digestion For High-throughput Proteomics Analyses -- [ Poster presented by Boston University Cardiovascular Proteomics Center at 2005 ASMS Annual Conference ]
  

For research use only. Not intended for use in diagnostic or therapeutic procedures.