In vitro systems for assessing the invasive potential of malignant as well as normal cells
 The BD BioCoat™ Matrigel™ Invasion Chambers are useful to study cell invasion of malignant and normal cells. The BD BioCoat Growth Factor Reduced (GFR) Matrigel Invasion Chambers can be used to study the mechanisms of invasion and to identify factors that interfere with this process in an in vitro environment. The Invasion Chambers consist of a BD Falcon ™ Cell Culture Insert with an 8 µm pore size PET membrane, uniformly coated with BD Matrigel Matrix, packaged ready-to-use in 6- and 24-well formats.
The BD Matrigel Matrix/GFR Matrigel Matrix serves as a reconstituted basement membrane in vitro. This matrix provides a true barrier to non-invasive cells while presenting an appropriate protein structure for invading cells to penetrate before passing through the membrane. The PET membrane is compatible with most fixatives and can be used with both light and electron microscopy. Processing is simplified as the durable membrane will not tear or curl and remains easy to handle when removed from the insert housing.
BD BioCoat Matrigel Invasion Chamber in the 6-well format (Cat. No. 354481) is well suited for selecting invasive cell phenotypes from non-invasive phenotypes in response to a chemoattractant. Post-invasion, the cells on the bottom of the membrane can be removed and used for further study.
Specific applications for the Matrigel Invasion Chamber include assessment of the metastatic potential of tumor cells1, 2 inhibition of metastasis by extracellular matrix components3, 4 or antineoplastic drugs5,6 altered expression of proteins in metastatic cells7,8,9,10,11,12 and invasion of normal cells, such as neutrophils13 eosinophils, endothelial cells. The BD BioCoat Matrigel Invasion Chamber has also been successfully used to study neutrophil transmigration through the basement membrane.14
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HT-1080 Cells
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Scanning electron micrograph of two human fibrosarcoma cells, having digested the BD Matrigel Matrix occluding the membrane and migrating through the 8 µm of the PET membrane. |
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- An in vitro system for assessing the invasive potential of both malignant and normal cells
- BD Matrigel Matrix serves as a model for the basement membrane and creates a barrier to noninvasive cells.
- Standard protocol includes procedure to quantify percent invasion
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Schematic drawing of BD BioCoat Matrigel Invasion Chamber in use.
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- BD BioCoat™ Matrigel™ Invasion Chamber--Guidelines for Use [ PDF ]
- BD BioCoat™ Matrigel™ Invasion Chambers and GFR Matrigel™ Invasion Chambers--Product Sheet [ PDF ]
References
- Albini, A. et al., Pathol. Oncol. Research, 406:532 (2003).
- Albini, A. et. al. Pathol. Oncol. Res. 406:532 (2000).
- Kobayshi, H. et al., Cancer Res. 52:3610 (1992).
- Yoshida, N., et. al., Br. J. Cancer, 80:1898 (1999).
- Melchiori, A. et al., Cancer Res. 52:2352 (1992).
- Parkkila, S., et al., Pro. Natl. Acad. Sci. USA, 97:92220 (2000).
- Sterms, M. and Want, M., Cancer Research, 47:2776 (1992).
- Clarke, E. A. et al., Nature, 406:532 (2000).
- Dohadwala et al., J. Biol. Chem., 276:20809 (2001).
- Graff, J. R. et al., J Biol. Chem., 275:2727 (2000).
- Jessani et al., Pro. Natl. Acad. Sci. USA, 99:10335 (2002).
- Kawai, N., et al., Gastroenterology 122:331 (2002).
- Delacourt, C. et. al., A.J. Respir. Cell Mol. Biol. 26:290 (2002).
- Keck, T., et al, Gastroenterology, 122(1): 188-201 (2002).
Remember to order BD BioCoat™ 8 µm Control Cell Culture Inserts when purchasing BD BioCoat Matrigel™ Invasion Chambers; 8 µm FluoroBlok Cell Culture Inserts when purchasing BD BioCoat FluoroBlok Invasion Chambers.
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