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This product is the replacement for 740971.
568225_2.png

Two-color flow cytometric analysis of CD24 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741) and with either BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 565032; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD24 antibody (Cat. No. 568225; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The bivariate pseudocolor density plot showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

568225Image1.png

Two-color flow cytometric analysis of CD24 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741) and with either BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 565032; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD24 antibody (Cat. No. 568225; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The bivariate pseudocolor density plot showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

568225_2.png 568225Image1.png Amber-Cap_Violet.png

Two-color flow cytometric analysis of CD24 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741) and with either BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 565032; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD24 antibody (Cat. No. 568225; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The bivariate pseudocolor density plot showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Two-color flow cytometric analysis of CD24 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741) and with either BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 565032; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD24 antibody (Cat. No. 568225; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The bivariate pseudocolor density plot showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Horizon™
Heat Stable Antigen Homologue (HSA); Ba-1; CD24A
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
IV B243; V CD24.5
AB_2916853
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  5. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  10. An isotype control should be used at the same concentration as the antibody of interest.
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568225 Rev. 1
Antibody Details
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ML5

The ML5 monoclonal antibody specifically binds to CD24 which is also known as CD24A, signal transducer CD24, small cell lung carcinoma cluster 4 antigen, or BA-1. CD24 is a 35-70 kDa glycophosphatidylinositol (GPI)-linked glycoprotein whose glycosylation pattern is highly variable and cell-type dependent. CD24 is expressed on neutrophils, eosinophils, dendritic cells, neural cells, epithelial cells, muscle cells, and many types of cancer cells. CD24 functions as an adhesion receptor. Several different ligands have been identified for CD24 including CD62P (P-selectin) which is expressed on activated platelets and activated endothelium. CD24 is variably expressed on all B lineage cells, except plasma cells, and can play a role in regulating the activation, proliferation or differentiation of these cells.

568225 Rev. 1
Format Details
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BV786
The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm.  BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter).  Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
altImg
BV786
407 nm
786 nm
568225 Rev.1
Citations & References
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View product citations for antibody "568225" on CiteAb

Development References (8)

  1. Chtanova T, Tangye SG, Newton R, et al. T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J Immunol. 2004; 173(1):68-78. (Clone-specific: Flow cytometry). View Reference
  2. Fang X, Zheng P, Tang J, Liu Y. CD24: from A to Z.. Cell Mol Immunol. 2010; 7(2):100-3. (Biology). View Reference
  3. Le Bien TW. CD24 Workshop Panel Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:539-541.
  4. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
  5. Pirruccello SJ, Bicak MS. Biochemical characterization of the CD24 antibody panel and cell-cycle studies of CD24 antigen expression on pre-B-ALL cell lines. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:83-84.
  6. Salamone MC, Rosselot C, Salamone GV, Barboza M, Kado M, Fainboim L. Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation . J Leukoc Biol. 2001; 69(2):215-223. (Clone-specific: Flow cytometry). View Reference
  7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  8. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
View All (8) View Less
568225 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.