BD Multitest™ CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP / CD19 APC reagent
Description
BD MultiTEST™ CD3 fluorescein isothiocyanate (FITC)/CD16+CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP)/CD19 allophycocyanin (APC) is a four-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T (CD3+), natural killer (NK) (CD3–CD16+CD56+), and B (CD3–CD19+) lymphocytes in erythrocyte-lysed whole blood. When used with TruCOUNT™ Tubes, absolute counts of these populations can be enumerated from a single tube. BD MultiTEST reagent and TruCOUNT Tubes can be used with the FACS Loader. BD MultiTEST reagent and TruCOUNT Tubes can be used with the FACS Loader.
Preparation And Storage
1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.
2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.
3. Store Trucount tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.
| Description | Quantity/Size | Part Number | Clone | Isotype |
|---|---|---|---|---|
| CD19 APC | N/A | 50-00126 | SJ25C1 | IgG1, κ |
| CD3 FITC | N/A | 50-7343 | SK7 | IgG1, κ |
| CD16 (FcγRIII) PE | N/A | 50-00116 | B73.1 | IgG1, κ |
| CD56 (NCAM-1) PE | N/A | 50-00117 | NCAM16.2 | IgG2b, κ |
| CD45 PerCP | N/A | 50-7464 | 2D1 | IgG1, κ |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.