BD^® Flex Single-Cell Multiplexing Kit

A species and cell type agnostic single-cell sample multiplexing tool to support pooling up to 24 samples in a BD Rhapsody™ System run

The BD^®Flex Single-Cell Multiplexing Kits (BD^®Flex SMK) utilize a two-step labeling approach to enable multiplexing for various types of single-cell samples. Every antibody-oligo in the BD^® Flex SMK has a unique sample oligo barcode conjugated to an anti-R-phycoerythrin (PE), clone E31-1459, antibody. This approach allows you to select any PE-conjugated primary antibody with which to tag your samples of interest, regardless of species or cell type.

Features

Support higher sample throughput for larger cohort studies	Multiplex up to 24 samples per lane to minimize batch effect, while maintaining high sample tagging sensitivity and specificity
Empower single-cell sample multiplexing for various species and cell types	BD^®Flex SMK enables flexibility in choosing primary antibodies with which to tag your samples
Reduce experimental cost with lower reagent cost per sample	Four separately sold kits each providing six samples tags take care of your small-scale study and help you save on the total BD Rhapsody^™System experiment when you multiplex more samples
One format fits all BD Rhapsody™ Assays	BD^®Flex SMK works seamlessly with BD Rhapsody^™Assays (transcriptome, cell surface protein, and immune repertoire profiling) on the BD Rhapsody^™ Single-Cell Analysis System
Increase cell number analyzed with improved data quality	Load up to 40K pooled cells per lane and remove erroneous data from cell multiplets originating from multiple samples for cleaner single-cell multiomics data analysis

Applications

Multiplexing 24 single-cell samples in one BD Rhapsody™ System run

t-SNE analysis of single-cell gene expression profile with 24 Sample Tags in the BD® Flex Single-Cell Multiplexing Kits and BD Rhapsody™ Human Immune Response Panel. Cells are clustered based on 399 genes in the BD Rhapsody™ Human Immune Response Panel. The three expected, phenotypically distinct population clusters corresponding to human B cell (Ramos, blue cells), T cell (Jurkat, yellow and green cells), and monocytes (THP1, red and brown cells) are obtained. High sample multiplexing sensitivity (>99%) and specificity (>95%) are achieved with 24 Sample Tags. Putative multiplet clusters are identified and removed by the BD® Flex SMK.