Training & eLearning

Welcome to the Introduction to Flow Cytometry Web-Based Training.

System Requirements

This course is best viewed using Internet Explorer® version 6 or later or Safari v3.2.1 or later, and Adobe® flash player 8.0 or later.

Course Objectives

  • Define key flow cytometry terms.
  • Describe and explain key flow cytometry principles.
  • Discuss the components and functions of the fluidics, optics, and electronics systems.
  • Explain how data is analyzed.
  • Describe the sorting process on flow cytometers.

Intro to Flow icon - Navigation 

Navigation

Learn how to navigate the training course.

Intro to Flow icon - Overview 

Overview

  • Define the term flow cytometry.
  • Describe the types of samples suitable for a flow cytometer.
  • Explain the properties that a flow cytometer can measure.
  • Identify the three subsystems of a flow cytometer.
Intro to Flow icon - Fluidics 

Fluidics

  • Describe the functions of the fluidics system.
  • Explain hydrodynamic focusing.
  • Describe how sample pressure affects flow rate and data resolution.
Intro to Flow icon - Optics 

Optics

  • Describe the functions of the optics system.
  • Discuss the role of lasers in BD cytometers.
  • Explain how filters route light to appropriate detectors.
Intro to Flow icon - Electronics 

Electronics

  • Describe how the electronics system converts light signals into numerical data.
  • Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
  • Explain how and why thresholds are used.
Intro to Flow icon - Optical Measurement 

Optical Measurements

  • Describe the factors that affect light scatter.
  • Explain how light scatter and fluorescence can be used to detect populations.
  • Describe the use of excitation and emission spectra.
  • Explain what compensation is and why it is necessary for multicolor analysis.
Intro to Flow icon - Data 

Data Analysis

  • Describe how data is saved and displayed.
  • Explain the purpose of gating.
  • State examples of data analysis methods.
Intro to Flow icon - Sorting 

Sorting

  • Describe the purpose of sorting cells or particles.
  • Explain how particle size affects the nozzle size and sheath pressure selection.
  • Describe the steps in droplet sorting.

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BD FACSCalibur™, BD FACSCanto™ II, and BD FACSCount™ are for In Vitro Diagnostic use.

BD FACSAria™ III, BD FACSArray ™, BD Influx™, BD™ LSR II, and BD LSRFortessa™ are for Research Use Only. Not for use in diagnostic or therapeutic procedures.

Class I (1) Laser Products.