The BD Horizon Brilliant™ Violet Reagent Portfolio and ASR Specificities for the Red and Blue Lasers Have Expanded.
BD continues to expand the analyte specific reagents (ASRs) portfolio, including the largest1 number of ASRs for use with the violet laser. Now we are increasing the BD Horizon Brilliant™ Violet ASR offering as well as adding new ASR specificities for the red and blue lasers.
- Expanded BD Horizon Brilliant™ Violet ASR portfolio increases flexibility for your multicolor panel designs. For use with flow cytometers that have violet lasers (405 nm).
- New specificities offer support for additional biological information on expanded clinical panels.
New ASRs
The following BD Horizon Brilliant™ ASRs are now available for consistency, brightness and channel flexibility.
| Description | Clone | ASR Cat. No. |
|---|---|---|
| CD138 BV421 | MI15 | 663984 |
| CD30 BV421 | BerH8 | 665128 |
| CD194 BV421 | 1G1 | 665128 |
| CD196 BV421 | 11A9 | 665130 |
| CD279 BV421 | MIH4 | 665131 |
| CD5 BV510 | L17F12 | 665132 |
| CD40 BV605 | 5C3 | 665133 |
| CD81 BV605 | JS-81 | 665134 |
| CD294 BV605 | BM16 | 665135 |
| CD366 BV605 | 7D3 | 665136 |
| CD27 BV510 | O323 | 665416 |
| CD42b PE | HIP1 | 665116 |
| CD152 PE | BNI3 | 665117 |
| CD183 PE-Cy7 | 1C6/CXCR3 | 665118 |
| CD185 APC | RF8B2 | 665119 |
| CD197 APC-R700 | 2-L1-A | 665120 |
| CD223 PerCP-Cy5.5 | T47-530 | 665121 |
| CD274 PE-Cy7 | MIH1 | 665123 |
| CD279 PE-Cy7 | MIH4 | 665124 |
| FoxP3 PE | 236A/E7 | 665125 |
| FoxP3 PerCP-Cy5.5 | 236A/E7 | 665126 |
| Ki-67 FITC | B56 | 665127 |
| CD194 BV421 | 1G1 | 665129 |
| CD196 BV421 | 11A9 | 665130 |
| CD279 BV421 | MIH4 | 665131 |
| CD40 BV605 | 5C3 | 665133 |
| CD294 BV605 | BM16 | 665135 |
| CD366 BV605 | 7D3 | 665136 |
Dye Profiles
BD Horizon Brilliant™ Violet 421 (BV421) Dye (Exmax 407 nm/Emmax 421 nm) is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Life Sciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates.
BD Horizon Brilliant™ Violet 510 (BV510) Dye is a tandem fluorochrome of the BD Horizon™ BV421 Dye and an acceptor dye with an emission maximum at 510 nm. The BD Horizon™ BV510 Dye offers a bright choice for the violet laser.
BD Horizon Brilliant™ Violet 605 (BV605) Dye (Exmax 407 nm/Emmax 602 nm) is a tandem fluorochrome that combines the BD Horizon™ BV421 Dye and an acceptor dye with emission at 602 nm. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates.
BD Horizon™ BV711 Dye is a tandem fluorochrome of the BD Horizon™ BV421 Dye and an acceptor dye with an emission maximum at 711 nm. This dye offers a bright choice for the violet laser typically as bright as PE on the blue laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the BD Horizon™ APC-R700 and PerCP-Cy5.5 detectors. BD Horizon™ BV711 dye will also have moderate spillover into the BD Horizon™ BV786 detector.
BD Horizon™ BV786 dye is a tandem fluorochrome of the BD Horizon™ BV421 dye and an acceptor dye with an emission maximum at 786 nm. The BD Horizon™ BV786 dye offers a bright choice for the violet laser typically as bright as PE on the blue laser.
Analyte Specific Reagent. Analytical and performance characteristics are not established.
Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
Pacific Blue is a trademark of Life Technologies Corporation.