BD Horizon™ Guided Panel Solution

Panel design can be a difficult and time-consuming process but is essential to obtaining good data. The BD Horizon™ Guided Panel Solution (GPS) provides a guided workflow for reagent selection based on the principles of panel design. This tool will help you streamline the panel design process and avoid reagent selections that may negatively affect population resolution.

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There are many factors to consider when designing a panel such as instrument configuration, fluorochrome brightness, spectral overlap, antigen density and co-expression and reagent availability. This can make panel design an overwhelming, iterative and time-consuming process. BD has created an online tool that organizes the principles of panel design into a guided workflow to help streamline and standardize the panel design process. Additionally, the tool has features to assess the reagent selections to help avoid selections that may negatively affect population resolution.

How the Tool Works

The panel design process has been broken into six steps. At each step, you will be prompted to input information that is pertinent to your panel. The information you will be prompted to enter is information that you likely use when designing panels currently. The tool has information about fluorochromes brightness, instrument configurations and resolution impact, so you do not have to enter that information. The tool takes all of this information into account to provide alerts for reagent selections that may negatively affect population resolution.

The more accurate the inputs are, the more likely the tool will be able to provide relevant outputs. It is also important to note that the tool will not be able to detect every possible reagent selection that may negatively affect resolution, nor will it recommend panels. Additionally, even good panels can have alert messages. This is especially true with higher order panels for which it may be difficult to avoid spectral overlap between reagents. In this case, the tool will allow you to see which populations are most likely to be negatively affected by the reagent selections and trade offs can be assessed.

  Panel Iteration 1 Panel Iteration 2 Panel Iteration 3
Panel CD127 FITC
CD4 PE
CD3 PerCP-Cy5.5
CD8 PE-Cy7
CD45RA APC
CD25 APC-Cy7
CD127 PE-Cy7
CD4 PerCP-Cy5.5
CD3 FITC
CD8 APC-Cy7
CD45RA APC
CD25 PE
CD127 Alexa Fluor® 647
CD4 APC-Cy7
CD3 FITC
CD8 PerCP-Cy5.5
CD45RA PE-Cy7
CD25 PE
BD Horizon GPS notifications of reagent selections that may affect population resolution
Table - Panel 1 Table - Panel 2 Table - Panel 3
Data Overview - Panel Table Plot 1
Mismatch of fluorochrome brightness and antigen resolution affects population resolution
Overview - Panel Table Plot 2
Fluorochrome brightness/antigen resolution rules applied, but spectral overlap on co-expressed markers prevents optimal resolution
Overview - Panel Table Plot 3
Principles taken into account and population is well resolved

How to Access the Tool

In order to use the tool, you will need to have an account on the bdbiosciences.com site. This allows you to save and manage panels to your account.

Providing Feedback

We wanted to share this tool as soon as possible, so we have released a beta version. We welcome feedback on the tool so we can make improvements in later versions.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures

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