BD ACCURI C6 PLUS
Cellular processes and events are probabilistic and take place in a context of complex varying environments, which they influence and which influence them. For this reason, flow cytometry is an ideal methodology for cell and cancer biology research since it supports the multiparametric analysis of individual cells and subpopulations in heterogeneous samples.
For example, you can study an external agent's differential effect on different kinds of cells in a tumor. You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. Results are rapid and resolve data at the single-cell level rather than averaging across a sample.
A personal flow cytometer in the lab provides many advantages for cell and cancer biology studies. When cells are ready for analysis or rare tumor samples arrive, it's crucial to have a flow cytometer at hand, ready to go. With two lasers and four fluorescence detectors, BD Accuri personal flow cytometers bring this versatility and convenience to your benchtop. Two additional features can further simplify your research:
Absolute counts. Cell concentration can be calculated directly from BD Accuri™ software statistics tables without the addition of counting beads.
Kits and templates. BD Biosciences offers a range of cost-effective cell biology kits ideally suited for the BD Accuri that include needed reagents and buffers to assess apoptosis, cell cycle and proliferation, and DNA damage. Free BD Accuri software templates simplify data collection and analysis.
The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications.
- White Paper: Life Science Application of Personal Flow Cytometry
- BD Accuri™ C6 Plus Brochure
- White Paper: The Essential Cell Analysis Tool
Apoptosis, Cell Cycle, DNA Damage, and Proliferation
Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. Detecting these events has broad application in the fields of cell biology, cancer research, and developmental biology.
BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. Apoptotic cells can be detected alone or simultaneously along with DNA damage and cell proliferation.
Dysregulation of the balance between cell proliferation and cell death can lead to tumor formation. Therefore, understanding the molecular signals that regulate cell cycle and apoptosis is key to the advancement of cancer research. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. These DNA intercalating agents and nucleotide analogs allow researchers to measure cellular DNA content, separate cells into G0/G1, S, and G2/M phases, analyze DNA ploidy, and detect DNA synthesis in actively dividing cells.
- Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer
- Product Information Sheet: BD Apoptosis Kits and Templates
- Product Information Sheet: BD Cell Cycle/DNA Kits and Templates
- Article: Assessing Mitochondrial Membrane Potential
- Webinar Replay: Fast, Accurate Apoptosis and Cell Cycle Studies Using Kits & Templates on the BD Accuri™ C6
- Customer Interview: Jeffrey Buis on Cell Cycle and DNA Research
Immunophenotyping is one of the foremost applications of flow cytometry due to its ability to recognize different cell types based on the expression of surface and intracellular proteins. In addition to apoptosis, cell cycle, and proliferation studies, immunophenotyping is essential for understanding the cellular ecology of cancer cells.
If you don’t yet know which proteins are typically expressed by a subpopulation of interest, BD Lyoplate™ cell surface marker screening panels provide a comprehensive and efficient solution for profiling cancer cells for hundreds of human or mouse cell surface markers by flow cytometry. Deciphering the cell surface proteome enables researchers to define strategies for the analysis and isolation of targeted cells from heterogeneous populations for functional studies, drug screening, and in vivo animal studies.
- Cancer Biology Applications on the BD Accuri™ C6 Plus
- Customer Interview: Suzanne Turner on Cancer Stem Cells
- Customer Interview: Yujie Liu on Cancer Stem Cells
- Customer Interview: Emmy Verschuren on Building Cancer Models
Cell signaling networks are critically involved in the regulation of cellular function. Cell survival, growth, and differentiation are tightly regulated through expression and post-translational modification of key proteins in signaling cascades.
Many common techniques for measuring cytokine signaling and protein phosphorylation, such as Western blot and ELISA, require cell lysis, which can not only obscure the differences among individual cells in a mixed population, but can also unintentionally activate signaling pathways. Flow cytometry provides a fast, accurate, and powerful method for investigating cell signaling. Researchers can use intracellular flow cytometry to assess production of key cytokines at the single-cell level. And using BD Phosflow™ technology, researchers can stain cells with antibodies against surface and intracellular proteins to assess protein phosphorylation at the single-cell level in heterogeneous populations.
- Product Information Sheet: BD Intracellular T-Cell Kits and Templates
- Product Information Sheet: Protein Phosphorylation Analysis Using Flow Cytometry
- Technical Bulletin: Studying Cell Signaling with the BD Accuri™ Flow Cytometer
- Webinar Replay: Enabling a Deeper Understanding of What is on, in, and Made by the Cell PDF | Video
Changes in intracellular calcium levels regulate many important cellular functions. Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a significant research challenge. To add test compounds to the cell suspension, a “stop-flow” method is often used in which sampling is paused, the sample tube opened, the agonist added, and the tube resealed. This technique leaves a gap or blind spot in data collection that may fail to capture essential changes in Ca2+ levels.
BD Accuri flow cytometers employ non-pressurized peristaltic pumps in an open fluidics system. Open tubes, such as Eppendorf® tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. This “continuous-flow” method enables nonstop monitoring of thousands of cells and accurate dynamic Ca2+ measurement of the entire population.
- White Paper: Continuous Measurement of Intracellular Calcium on the BD Accuri™ Flow Cytometer
- Webinar Slideset: Continuous Measurement of Intracellular Calcium and Other Cellular Responses
Screening thousands of cells for reporter gene expression levels is fundamental to understanding how genes are regulated inside the cell. BD Accuri systems can help you quickly examine reporter gene transfection efficiency and cell population heterogeneity—right from your benchtop. You can add additional reagents to screen cell populations, monitor DNA content, identify cells at different phases of the cell cycle, and examine heterogeneity and transient effects in reporter expression.
BD Accuri systems can measure the expression of Green, Yellow, or Red Fluorescent Protein (GFP, YFP, or RFP), as well as other fluorescent proteins, in their standard configurations. Optional filters may be used to detect signals more clearly or to resolve two fluorescent proteins with overlapping emission spectra.
- Product Information Sheet: Detecting Red Fluorescent Proteins on the BD Accuri™
- Technical Bulletin: Optical Filter Guidelines for Fluorescent Protein Analysis with the BD Accuri™ Flow Cytometer
- Technical Bulletin: Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy with the BD Accuri™ Flow Cytometer
- Webinar Replay: Coupling Flow Cytometry with an Expanded Palette of Fluorescent Proteins to Enhance Gene Expression Analysis
- Customer Interview: Timothy Cooper on Using Gene Expression to Study Bacterial Evolution
Researchers are increasingly using flow cytometry to measure kinetic cellular processes that change over time, such as cellular response to an external event or stimulus. A new method, called continuous or “real-time” flow cytometry, involves adding an agent to a sample while simultaneously analyzing it.
The real-time method can measure almost any dynamic process and is broadly applicable in cell biology and many other fields of research. Applications include cell death, erythrocyte lysis, nanoparticle uptake, platelet activation, GFP expression, optimization of staining protocols, and disinfection of drinking water. The method has the potential to dramatically increase data richness and shed light on processes that would be difficult to assess by capturing and analyzing samples at discrete intervals.
An open, non-pressurized fluidics system makes BD Accuri systems ideal for kinetic studies. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. The real-time method enables nonstop monitoring of thousands of cells and accurate dynamic measurement of the entire population.
- Webinar Replay: Cell Biology Applications of Real-Time Flow Cytometry
- Customer Interview: Alfonso Blanco-Fernández on Real-Time Flow Cytometry
All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends.