PE Mouse Anti-Human CD79b

This is a suggested alternate SKU for 561943. Contact us for additional support.

BD Pharmingen™ PE Mouse Anti-Human CD79b

Name: PE Mouse Anti-Human CD79b
Brand: BD Pharmingen™
SKU: 555679

Clone CB3-1

(RUO)

Two-color flow cytometric analysis of CD79b expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD79b (Cat. No. 555679/561943) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 555899).The two color fluorescence dot plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Ig-beta; IGB; B29

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Purified CD79αβ from Ramos B Cell Line

Application: Flow cytometry (Tested During Development)

Vol. Per Test: 20 µl

Workshop Number: VI CD79.1

Entrez Gene ID: 974

RRID: AB_396032

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

PE Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555749

FITC Mouse Anti-Human CD19 RUO

Size 100 Tests Cat No. 555412

View Details

555679 Rev. 9

Antibody Details

CB3-1

Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Igα and Igβ, which have been designated in the Fifth International Leukocyte Workshop as CD79a and CD79b respectively. The CB3-1 monoclonal antibody specifically binds to CD79b, which is expressed on surface Ig (sIg)-positive lymphocytes and B-cell lines but only in the cytoplasm of sIg-negative cells including most terminal deoxynucleotidyl transferase (TdT) positive early pre-B and all cytoplasmic µ positive pre-B cell lines. Antibodies to CD79b are helpful in delineating signal transduction pathways activated via antibody receptors during different stages of B-cell differentiation.

555679 Rev. 9

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

555679 Rev.9

Citations & References

Development References (6)

Nakamura T, Kubagawa H, Cooper MD. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992; 89(18):8522-8526. (Biology). View Reference
Nakamura T, Sekar MC, Kubagawa H, Cooper MD. Signal transduction in human B cells initiated via Ig beta ligation.. Int Immunol. 1993; 5(10):1309-15. (Clone-specific). View Reference
Nakamura T. CD79 Workshop Panel Report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:180-182.
Sanchez M, Misulovin Z, Burkhardt AL. Signal transduction by immunoglobulin is mediated through Ig alpha and Ig beta. J Exp Med. 1993; 178(3):1049-1055. (Biology). View Reference
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Zabel BA, Nakae S, Zúñiga L, et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008; 205(10):2207-2220. (Immunogen). View Reference

View All (6)

555679 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.