Recombinant Human p53 Protein (Wildtype)

Our newest Spectracomp™ Unmixing and Compensation Particles (Cat. No. 554294) offer both positive and negative beads against mouse, rat, and hamster isotypes in one convenient vial. TESTING

BD Pharmingen™ Recombinant Human p53 Protein (Wildtype)

Name: Recombinant Human p53 Protein (Wildtype)
Brand: Recombinant Human p53 Protein (Wildtype)
SKU: 556439

Protocol Library

Scientific Resources

Protocol Library

Scientific Resources

Product Details

Description

p53 is a nuclear phosphoprotein that plays a key role in cell growth regulation, particularly inhibition of cell proliferation. BD Biosciences Pharmingen offers a panel of p53 specific antibodies. The recombinant p53 protein is useful as a western blot control for antibodies that recognize human p53 and work in western blot. These antibodies include the following p53 clones: G59-12, PAb 122, PAb 240, PAb 1801, DO-1, DO-7. Recombinant human p53 was expressed in the Baculovirus Expression Vector System (BEVS) and purified from Sf9 insect cell lysate using an anti-p53 antibody, clone DO-7 (Cat. No. 554294).

The purity and the integrity of the protein was verified by SDS-PAGE and Western blot analysis.

Preparation And Storage

This preparation contains no preservatives, thus it should be handled under aseptic conditions. Avoid multiple freeze-thaws of product.

The protein solution was sterile filtered and frozen at -20°C. Store the protein at -20°C; it may also be stored at -80°C.

Recommended Assay Procedures

1 µg of protein can easily be visualized on a Coomassie Blue stained SDS/PAGE as a band of 53 kDa. For Western blot analysis, 50 ng or less is sufficient for the chemiluminescence detection method. The p53 recombinant protein has not been evaluated for suitability in other assays outside of Western blot analysis.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

Purified Mouse Anti-Human p53 RUO

Size 0.1 mg Cat No. 554293

Purified Mouse Anti-Human p53 RUO

Size 0.1 mg Cat No. 554157

Purified Mouse Anti-p53 RUO

Size 0.1 mg Cat No. 554147

Purified Mouse Anti-p53 RUO

Size 0.1 mg Cat No. 554166

Purified Mouse Anti-Human p53 RUO

Size 0.1 mg Cat No. 554294

556439 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.