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A78720_611083_IMAGE1.png

Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

A78720_611083_image2.png

Immunofluorescence staining of human endothelial cells.

A78720_611083_IMAGE1.png A78720_611083_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of AIM-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- AIM-1 antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Aurora B; Aurora and Ipl1-like midbody associated protein
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Rat AIM-1 aa. 2-124
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
41 kDa
250 µg/ml
AB_398396
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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611082 Rev. 2
Antibody Details
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6/AIM-1

The mitotic phase of the cell cycle is a complex process that ensures the fidelity of chromosome segregation. During the final stage of mitosis (telophase), segregated chromosomes become less condense and nuclear membranes surround the two sets of daughter chromosomes. Simultaneously, the separation and segregation of the cytoplasm (cytokinesis) ensures complete division and formation of two identical daughter cells. Regulation of cytokinesis is poorly understood and errors in this process can lead to cell death or oncogenesis. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae Ipl1 kinase are highly homologous and are required for progression through mitosis. Their mammalian homolog AIM-1 (also known as Aurora and Ipl1-like midbody associated protein) accumulates at the G2/M interface. During late anaphase, AIM-1 is found at the equator of central spindles. However, during telophase and cytokinesis, it is found at the midbody. Although over-expression of a kinase-inactive AIM-1 mutant disrupts formation of the cleavage furrow, nuclear division is unaffected. Thus, it is thought that AIM-1 is essential for cleavage furrowing and the onset of cytokinesis.

611082 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611082 Rev.2
Citations & References
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View product citations for antibody "611082" on CiteAb

Development References (5)

  1. Chen J, Jin S, Tahir SK. Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells. J Biol Chem. 2003; 278(7):486-490. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  2. Lange BM, Rebollo E, Herold A, Gonzalez C. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes. EMBO J. 2002; 21(20):5364-5374. (Biology: Immunoprecipitation). View Reference
  3. Tatsuka M, Katayama H, Ota T. Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res. 1998; 58(21):4811-4816. (Biology). View Reference
  4. Terada Y, Tatsuka M, Suzuki F, Yasuda Y, Fujita S, Otsu M. AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 1998; 17(3):667-676. (Biology). View Reference
  5. Trinkle-Mulcahy L, Andrews PD, Wickramasinghe S, et al. Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle. Mol Biol Cell. 2003; 14(1):107-117. (Biology: Immunofluorescence). View Reference
View All (5) View Less
611082 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.