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Purified Mouse Anti-β-Arrestin
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat (Tested in Development)
Mouse IgG1
Rat β-Arrestin1 aa. 262-409
Western blot (Routinely Tested), Immunofluorescence (Reported), Immunohistochemistry, Immunoprecipitation (Not Recommended)
55 kDa
250 µg/ml
AB_397907
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610551 Rev. 1
Antibody Details
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10/Beta-Arrestin1

β-Arrestins were discovered due to their ability to modulate interactions between the phosphorylated β2-Adrenergic receptors and G proteins. This modulation results in diminished β2-Adrenergic receptor function, also known as desensitization. Because arrestins are found at the synaptic terminals, they may provide a termination mechanism that allows the neurons to regain their original polarization and respond to a new neurotransmitter stimulus. The C-terminal region of arrestins is involved in selecting the phosphorylated and activated adrenergic receptors. The β-Arrestin1 gene encodes a protein of 418 amino acids with an approximate molecular weight of 55kDa. β-Arrestin1 protein is highly homologous to the 45kDa β-Arrestin2. Both proteins are widely expressed, but are especially abundant in the central nervous system.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610551 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610551 Rev.1
Citations & References
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Development References (5)

  1. Attramadal H, Arriza JL, Aoki C, et al. Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family. J Biol Chem. 1992; 267(25):17882-17890. (Biology). View Reference
  2. Dalle S, Imamura T, Rose DW, et al. Insulin induces heterologous desensitization of G-protein-coupled receptor and insulin-like growth factor I signaling by downregulating beta-arrestin-1. Mol Cell Biol. 2002; 22(17):6272-6285. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  3. DeFea KA, Zalevsky J, Thoma MS, Dery O, Mullins RD, Bunnett NW. beta-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J Cell Biol. 2000; 148(6):1267-1281. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Gurevich VV, Dion SB, Onorato JJ, et al. Arrestin interactions with G protein-coupled receptors. Direct binding studies of wild type and mutant arrestins with rhodopsin, beta 2-adrenergic, and m2 muscarinic cholinergic receptors. J Biol Chem. 1995; 270(2):720-731. (Biology). View Reference
  5. Imamura T, Huang J, Dalle S, et al. beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport. J Biol Chem. 2001; 276(47):43663-43667. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610551 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.