Description
BD Simultest™ Control γ1/γ2a (IgG1 FITC/IgG2a PE) is a two-color direct immunofluorescence reagent for use as a negative control in flow cytometric immunophenotyping of erythrocyte-lysed whole blood (LWB). BD Simultest Control γ1/γ2a is used with BD Simulset™ software to set fluorescence-1 (FL1) and fluorescence-2 (FL2) quadrant markers around the unstained (negative) lymphocyte population and to estimate the amount of nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors. BD Simultest Control γ1/γ2a is for in vitro diagnostic use with BD Simultest in vitro diagnostic reagents.
Preparation And Storage
• BD Simultest Control γ1/γ2a is for in vitro diagnostic use with BD Simultest in vitro diagnostic reagents.
• When stored at 2° to 8°C, antibody reagents are stable until the expiration date shown on the label. Do not use after the expiration date.
• The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells.
• Incubation or centrifugation times or temperatures other than those specified may be a source of error.
• For optimal results, stain blood samples within 6 hours of venipuncture.
• Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.
• The antibody reagents contain sodium azide as a preservative. However, care should be taken to avoid microbial contamination, which may cause erroneous results.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| IgG2a PE | X39 | IgG2a, | N/A |
| Mouse IgG1 Isotype Control FITC | X40 | IgG1, κ | N/A |
Development References (8)
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A National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991. (Biology).
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Anderson CL, Looney RJ. Human leukocyte IgG Fc receptors. Immunol Today. 1986; 9:264-266. (Biology).
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Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
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Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
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Landay AL, Muirhead KA. Procedural guidelines for performing immunophenotyping by flow cytometry. Clin Immunol Immunopath. 1989; 52:48-60. (Biology).
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McCarthy RC, Fetterhoff TJ. Issues for quality assurance in clinical flow cytometry. Arch Pathol Lab Med. 1989; 113:658-666. (Biology).
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National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. 1992; 1992. (Biology).
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Patrick CW, Swartz SJ, Harrison KA, Keller RH. Collection and preparation of hematopoietic cells for cell marker analysis. Lab Med. 1984; 15:659-665. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.