BD OMICS-One™ Splint Oligonucleotide Removal Buffer

The BD® OMICS-One Splint Oligonucleotide Removal Buffer has been optimized for ATAC-Seq assays on the BD Rhapsody™ Single-Cell Analysis System. This buffer is used in the removal of unused splint oligonucleotides from the BD Rhapsody™ Enhanced Cell Capture Beads v3 prior to Exonuclease I treatment, reducing noise that may arise from residual oligonucleotides and ensuring optimal assay performance.

In multiomic ATAC-Seq + Whole Transcriptome Analysis (WTA) workflow, the buffer is used after the reverse transcription step to remove unused splint oligonucleotides that were added to the beads for capturing genomic DNA fragments, making bead-bound oligonucleotides accessible for subsequent Exonuclease I treatment. In multiplexed standalone ATAC-Seq workflow, the buffer is applied after the gap filling step, which is performed following the ligation of tagmented DNA fragments to the beads to fill the gaps and extend bound fragments as well as generate the complementary strand of the captured Sample Tag molecules on the beads' dT capture sites. In multiplexed ATAC-Seq + WTA workflow, the buffer is used after the reverse transcription step, which is performed to achieve gap filling of the genomic fragments captured on the beads and generate the complementary DNA and second strand from the captured RNA and Sample Tag molecules, respectively. This buffer is identical to the reagent with the same label (catalog number  51-9024041) included in the BD Rhapsody™ Multiomic ATAC-Seq Amplification Kit (catalog number 571361) but is provided in a larger volume to ensure a sufficient supply particularly for multiplexed workflows.

For recommended assay procedures covering multiomic ATAC-Seq and multiplexing workflows with either standalone ATAC-Seq or multiomic ATAC-Seq assays, refer to the protocols listed in https://scomix.bd.com/ or https://www.bdbiosciences.com/en-us/resources/protocols/single-cell-multiomics.