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Leverage single-cell epigenomics for immunology discovery
Multimodal profiling can help us gain new insights about immune biology. This webcast will describe an innovative, multimodal approach to T cell functional phenotyping by combining spectral image-enabled cell sorting with single-cell multiomics covering whole transcriptome and epigenomic profiling using an ATAC-seq assay (assay for transposase-accessible chromatin using sequencing). This multimodal approach was used to analyze ~200,000 T cells sorted from an in vitro model of T cell activation to reveal in-depth molecular and cellular dynamics data about T cell activation and biology.
Our expert speakers will walk you through a step-by-step approach comprising experimental design, methods, analyses and data covering the discovery process in detail. They will also discuss the end-to-end workflow from sample prep to bioinformatic analyses of transcription factor engagement at the single-cell level so that you are well positioned to implement these cutting-edge approaches in your research.
Learn how to:
- Utilize single-cell ATAC-seq and whole transcriptome assays for immunology research
- Combine image-based spectral cell sorting with single-cell multiomics to decipher gene regulation and transcription factor biology
- Further discovery in immunology with single-cell multiomics
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