PE Mouse Anti-Human IDH1
Clone RMab-3 (RUO)
- Brand BD Pharmingen™
- Alternative Name isocitrate dehydrogenase [NADP] cytoplasmic; IDH; IDP; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; PICD
- Concentration 0.2 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing) Rat, Mouse, Chinese Hamster (Reported)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human IDH1-R132H Peptide
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The RMab-3 monoclonal antibody specifically recognizes wild type and mutant forms of isocitrate dehydrogenase 1 (IDH1). IDH1 is a NADP(+)-dependent enzyme that is found as a homodimer in the cytoplasm and peroxisomes. It catalyzes the conversion of isocitrate to alpha-ketoglutarate via a redox reaction, which is thought to be the primary mechanism that produces the NADPH that is required for cholesterol and fatty acid biosynthesis and for the generation of reduced glutathione that prevents cellular damage from reactive oxygen species. Consistent with IDH1's participation in multiple metabolic pathways, IDH1 mRNA has been detected in all normal tissues and cancers. A mutant form of IDH1 that frequently occurs in several forms of gliomas and secondary glioblastomas correlates with greater invasive behavior of gliomas. That same mutation may also contribute to leukemic transformation.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.