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PE Mouse Anti-Human IDH1 RMab-3 RUO

PE Mouse Anti-Human IDH1 

Clone  RMab-3   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name isocitrate dehydrogenase [NADP] cytoplasmic; IDH; IDP; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; PICD
  • Concentration 0.2 mg/ml
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing)  Rat, Mouse, Chinese Hamster (Reported) 
  • Application Intracellular staining (flow cytometry) (Routinely Tested) 
  • Immunogen Human IDH1-R132H Peptide
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The RMab-3 monoclonal antibody specifically recognizes wild type and mutant forms of isocitrate dehydrogenase 1 (IDH1). IDH1 is a NADP(+)-dependent enzyme that is found as a homodimer in the cytoplasm and peroxisomes. It catalyzes the conversion of isocitrate to alpha-ketoglutarate via a redox reaction, which is thought to be the primary mechanism that produces the NADPH that is required for cholesterol and fatty acid biosynthesis and for the generation of reduced glutathione that prevents cellular damage from reactive oxygen species. Consistent with IDH1's participation in multiple metabolic pathways, IDH1 mRNA has been detected in all normal tissues and cancers. A mutant form of IDH1 that frequently occurs in several forms of gliomas and secondary glioblastomas correlates with greater invasive behavior of gliomas. That same mutation may also contribute to leukemic transformation.

Format
  • Format PE
  • Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
  • Excitation Max 496 nm
  • Emission Max 578 nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Suggested Companion Products

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

Fixation Buffer RUO
100 mL Cat No: 554655

Perm/Wash Buffer RUO
100 mL Cat No: 554723

PE Mouse IgG1, κ Isotype Control RUO
0.1 mg Cat No: 554680

Lyse/Fix Buffer 5X RUO
250 mL Cat No: 558049

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.


BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.


  1. Abbas S, Lugthart S, Kavelaars FG, et al. Acquired mutations in the genes encoding IDH1 and IDH2 both are recurrent aberrations in acute myeloid leukemia: prevalence and prognostic value.. Blood. 2010; 116(12):2122-6. (Biology: Intracellular staining (flow cytometry)). View reference

  2. Bogdanovic E. IDH1, lipid metabolism and cancer: Shedding new light on old ideas. Biochim Biophys Acta. 2015; 1850(9):1781-5. (Biology: Intracellular staining (flow cytometry)). View reference

  3. Cairns RA, Mak TW. Oncogenic isocitrate dehydrogenase mutations: mechanisms, models, and clinical opportunities.. Cancer Discov. 2013; 3(7):730-41. (Biology: Intracellular staining (flow cytometry)). View reference

  4. Kato Y, Natsume A, Kaneko MK. A novel monoclonal antibody GMab-m1 specifically recognizes IDH1-R132G mutation.. Biochem Biophys Res Commun. 2013; 432(4):564-7. (Clone-specific: Intracellular staining (flow cytometry)). View reference

  5. Losman JA, Looper RE, Koivunen P, et al. (R)-2-hydroxyglutarate is sufficient to promote leukemogenesis and its effects are reversible.. Science. 2013; 339(6127):1621-5. (Biology: Intracellular staining (flow cytometry)). View reference

  6. Metallo CM, Gameiro PA, Bell EL, et al. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia.. Nature. 2011; 481(7381):380-4. (Biology: Intracellular staining (flow cytometry)). View reference

  7. Patil NK, Bohannon JK, Hernandez A, Patil TK, Sherwood ER. Regulation of leukocyte function by citric acid cycle intermediates.. J Leukoc Biol. 2019; 106(1):105-117. (Biology: Intracellular staining (flow cytometry)). View reference

  8. Shechter I, Dai P, Huo L, Guan G. IDH1 gene transcription is sterol regulated and activated by SREBP-1a and SREBP-2 in human hepatoma HepG2 cells: evidence that IDH1 may regulate lipogenesis in hepatic cells.. J Lipid Res. 2003; 44(11):2169-80. (Biology: Intracellular staining (flow cytometry)). View reference

  9. Takano S, Kato Y, Yamamoto T, et al. Immunohistochemical detection of IDH1 mutation, p53, and internexin as prognostic factors of glial tumors.. J Neurooncol. 2012; 108(3):361-73. (Immunogen: Intracellular staining (flow cytometry)). View reference

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