Perm/Wash Buffer I
- Brand BD Phosflow™
Intracellular staining (flow cytometry) (Routinely Tested)
- Storage Buffer Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins. BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies. BD Phosflow™ Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
Do not freeze.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Flow cytometry: Dilute BD Phosflow™ Perm/Wash Buffer I 1:10 in distilled water prior to use. If desired, the diluted 1X BD™ Phosflow Perm/Wash Buffer I can be passed through a 0.45 µm filter to remove any residual precipitate. The BD Phosflow™ Perm/Wash Buffer I can be used to permeabilize or wash cells and to dilute antibodies for immunofluorescent staining of intracellular proteins.