Human Regulatory T Cell Cocktail
- Brand BD Pharmingen™
Flow cytometry (Tested During Development)
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Cocktail Component Clone Isotype
FITC anti-Human CD4 SK3 mIgG1, κ
PE-Cy7 anti-Human CD25 2A3 mIgG1, κ
Alexa Fluor® 647 anti-Human CD127 HIL-7R-M21 mIgG1, κ
The Human Regulatory T cell cocktail is a three color reagent used to identify the natural T regulatory cell (nTreg) population. An expression pattern of CD4+CD25int/brightCD127dim closely correlates to the expression of the transcription factor Forkhead box P3 (FoxP3).FoxP3 is considered a specific marker to T regulatory cells (Tregs). The SK3 antibody recognizes CD4, an antigen expressed primarily on helper/inducer T lymphocytes, although this antigen is also present in low density on monocytes. The 2A3 antibody recognizes CD25, an antigen also known as the human low-affinity interleukin-2 receptor (IL-2R). The CD25 antigen is present on a subset of peripheral blood lymphocytes. Antigen density increases on phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes; T lymphocytes from mixed lymphocyte cultures; and human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines. hIL-7R-M21 reacts with the 60 - 90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα). The receptor is a heterodimer composed of the CD127 and the common gamma chain, shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, and IL-15R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
For an Application Note on how to isolate and characterize regulatory T cells, please refer to the following link: