Purified Rat Anti-Mouse CD45
Clone 30-F11 (RUO)
- Brand BD Pharmingen™
- Alternative Name Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4
- Concentration 0.5 mg/ml
- Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
Immunoprecipitation, Immunochemistry, Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-zinc-fixed, Cytotoxicity, Immunofluorescence (Reported)
- Immunogen Mouse Thymus / Spleen
- Entrez Gene ID 19264
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The polyclonal antibody was purified from antiserum by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
This antibody has been tested by immunofluorescent staining (≤ 1 µg/million cells) by flow cytometric analysis. For Immunohistochemical staining, we recommend the use of purified 30-F11 mAb in our special formulation for IHC, Cat. No. 550539.