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PE Mouse anti-Rabbit IgG Detector C101-359 RUO

PE Mouse anti-Rabbit IgG Detector 

Clone  C101-359   (RUO)

  • Brand BD™ Cytometric Bead Array (CBA)
  • Vol. Per Test 50 µl
  • Isotype Mouse BALB/c IgG1, κ
  • Application Flow cytometry (Routinely Tested) 
  • Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The PE Mouse anti-Rabbit IgG Detector is useful in verification of the conjugation of rabbit IgG isotype antibodies to the BD CBA Functional Beads. It does not react with rabbit IgM. It does not cross-react with mouse, rat, human or guinea pig immunoglobulins.

Format
  • Format PE
  • Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
  • Excitation Max 496 nm
  • Emission Max 578 nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Other Formats →

Suggested Companion Products

Functional Bead Conjugation Buffer Set RUO
15 Tests Cat No: 558556

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This conjugated product is sold under license to the following patents: US Patent No. 5,798,276.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


This monoclonal antibody has been formulated at 50 µl/test and is useful in detection of rabbit IgG antibody conjugated to the BD CBA Functional Beads. For specific instructions on the use of this antibody for the detection of antibody conjugated to BD CBA Functional Beads, refer to the Conjugation Confirmation procedure in the BD CBA Functional Bead Conjugation Buffer Set Instruction Manual (Cat. No. 558556).

558553 Rev. 2
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