TMB Substrate Reagent Set
- Brand BD OptEIA™
ELISA (Tested During Development)
- Regulatory Status RUO
Regulatory Status Legend
The TMB substrate reagent set is designed for use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is a colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.
|51-2606KC||Substrate Reagent A||300 mL (1 ea)|
|51-2607KC||Substrate Reagent B||300 mL (1 ea)|
Preparation and Storage
Store unopened reagents at 2-8°C. Do not use after the expiration date. Discard any remaining working solutions after use. Reagents should be brought to room temperature (20-25°C) prior to use. Allow at least 30 minutes for this process. Avoid prolonged exposure to light, contact with metal, air or extreme temperatures as color may develop.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Prepare a working concentration of TMB substrate solution within 15 minutes prior to use by mixing equal volumes of Substrate Reagent A and Substrate Reagent B (e.g. for one 96-well plate, a 12 mL TMB substrate working solution can be prepared by mixing 6 mL of Substrate A with 6 mL of Substrate Reagent B). When mixed together, TMB substrate solution should be colorless to a very faint blue. In the presence of peroxide-labeled conjugates, the solution should develop a definitive blue color. After the addition of sulfuric or phosphoric acid to stop the reaction, the color changes from blue to yellow.
Danger: Substrate Reagent B (component 51-2607KC) contains 33.05% methanol (w/w), 13.34% glycerin (w/w), and 1.745% DMSO (w/w).
Flammable liquid and vapour.
Toxic if swallowed, in contact with skin or if inhaled.
Causes damage to the central nervous system. Route of exposure: Oral.
Causes damage to oragns.
Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
Keep container tightly closed. Ground and bond container and receiving equipment. Use explosion-proof [electrical/ventilating/lighting/] equipment. Use non-sparking tools. Take action to prevent static discharges. Use suitable extinguishing media to extinguish.
Wear protective gloves / eye protection.
Wear protective clothing.
Do not breathe mist/vapours/spray.
Use only outdoors or in a well-ventilated area.
Wash thoroughly after handling. Do not eat, drink or smoke when using this product.
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.Take off immediately all contaminated clothing. Wash contaminated clothing before reuse.
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
IF SWALLOWED: Call a POISON CENTER/doctor/ if you feel unwell. Rinse mouth.
IF exposed or concerned: Call a POISON CENTER/doctor/
Store in a well-ventilated place. Keep cool. Store locked up. Keep container tightly closed.
Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.