Purified Mouse Anti-BRUCE
Clone 4/BRUCE (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name BIR Repeat containing Ubiquitin-Conjugating Enzyme
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing) Mouse, Rat, Dog (Tested in Development)
Western blot (Routinely Tested)
Immunofluorescence (Tested During Development)
- Immunogen Mouse BRUCE aa. 372-571
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
- Format Purified
Suggested Companion Products
Preparation and Storage
Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Western blot: Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp
Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (Cat. No. 611449)