Purified NA/LE Anti-Human TNF Adalimumab Biosimilar

BD Pharmingen™ Purified NA/LE Anti-Human TNF Adalimumab Biosimilar

Name: Purified NA/LE Anti-Human TNF Adalimumab Biosimilar
Brand: Purified NA/LE Anti-Human TNF Adalimumab Biosimilar
SKU: 569601

Clone Adalimumab.rMAb (also known as Adalimumab Biosimilar.rMAb) (RUO)

Neutralization of cytotoxicity induced by Human TNF with increasing doses of Purified NA/LE Human Anti-Human TNF (Adalimumab Biosimilar) antibody. Cells from the Mouse L-929 (Mouse Fibroblast ATCC® CCL-1™) cell line were cultured with BD Pharmingen™ Recombinant Human TNF protein (BD Cat. No. 554618; at 0.3125 ng/ml), the metabolic inhibitor Actinomycin D (ThermoFisher Cat. No. A7592; 0.5 µg/ml) and increasing doses of Purified NA/LE Human Anti-Human TNF (Adalimumab Biosimilar) antibody. After culture for 24 hours, cell viability was measured by using a colorimetric MTT assay.

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Catalog No:569601

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0.5 mg

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Product Details

Brand: BD Pharmingen™

Alternative Name: TNF; TNF-a; TNF-alpha; TNFA; TNFSF2

Reactivity: Human (QC Testing)

Isotype: Human IgG1, κ

Immunogen: Human TNF

Application: ELISA (Routinely Tested), Neutralization (Tested During Development)

Concentration: 1.0 mg/ml

Entrez Gene ID: 7124

RRID: AB_3685172

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2 µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
An isotype control should be used at the same concentration as the antibody of interest.

Data Sheets

Technical Data Sheet (TDS)

Safety Data Sheet

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Recombinant Human TNF RUO

Size 10 µg Cat No. 554618

569601 Rev. 1

Antibody Details

Adalimumab.rMAb

The Adalimumab.rMAb (also known as Adalimumab Biosimilar.rMAb) is a research grade recombinant monoclonal antibody that specifically recognizes Tumor Necrosis Factor (TNF) similarly to therapeutic Adalimumab. Therapeutic Adalimumab is a recombinant human IgG1 monoclonal human TNF-specific antibody used in the treatment of a wide variety of inflammatory conditions such as rheumatoid arthritis, ankylosing spondylitis, Crohn's disease, septic shock, and cancer. Adalimumab specifically binds to TNF and neutralizes its biological activity by blocking its binding to its cell surface receptors. Adalimumab does not bind to or inactivate lymphotoxin (Tumor necrosis factor-beta). TNF, also known as TNF alpha (TNF-α) or Cachectin, is an ~26 kDa type II transmembrane protein that is encoded by TNF and serves as the prototypic ligand of the TNF superfamily. TNF is a multifunctional cytokine that plays roles in inflammation, immune system development, innate and adaptive immune responses, apoptosis, lipid metabolism, and necrosis of some tumor cells. TNF is variably produced by a wide variety of activated leucocytes, epithelial cells, endothelial cells, and tumor cells. This mediator is expressed as a noncovalently bound homotrimer that is expressed on the cell surface. Membrane TNF is enzymatically cleaved from the cell surface by tumor necrosis factor alpha converting enzyme (TACE), also known as ADAM17 and CD156b, into a soluble biologically active trimer of the TNF extracellular domains. TNF mediates its biological activities through binding to cell surface TNF Receptor Type 1 (TNFR1, CD120a) and Type 2 (TNFR2, CD120b), which are widely expressed on normal hemopoietic and nonhemopoietic cells as well as tumor cells.

The Adalimumab.rMAb is intended for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals.

569601 Rev. 1

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

569601 Rev.1

Citations & References

Development References (4)

Grewal IS. Overview of TNF superfamily: a chest full of potential therapeutic targets. Adv Exp Med Biol. 2009; 647:1-7. (Biology). View Reference
Ingelman-Sundberg HM, Laestadius Å, Chrapkowska C, et al. Diverse effects on vaccine-specific serum IgG titres and memory B cells upon methotrexate and anti-TNF-α therapy in children with rheumatic diseases: A cross-sectional study.. Vaccine. 2016; 34(10):1304-11. (Biology). View Reference
Meager A. Assays for cytotoxicity.. Methods Mol Biol. 2004; 249:135-52. (Methodology). View Reference
Ward-Kavanagh LK, Lin WW, Šedý JR, Ware CF. The TNF Receptor Superfamily in Co-stimulating and Co-inhibitory Responses.. Immunity. 2016; 44(5):1005-19. (Biology). View Reference

View All (4)

569601 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.