Multicolor flow cytometric analysis of adult mouse spleen cells and bone marrow hematopoietic stem cells. (Panel A) BALB/c spleen cells were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092) and PE Rat Anti-Mouse CD150 (Cat. No. 562651), staining cells well above background compared to PE Rat IgG2a, κ isotype control (Cat. No. 557690) (data not shown). A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by events with the forward and side-light scattering characteristics of viable lymphocytes. (Panel B) BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE-Cy™7 Hamster Anti-Mouse CD48 (Cat. No. 560731), and PE Rat Anti-Mouse CD150 antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P4 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated). Lineage-negative and CD48- CD150+ (Middle Plot, P3 Gate) bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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Multicolor flow cytometric analysis of adult mouse spleen cells and bone marrow hematopoietic stem cells. (Panel A) BALB/c spleen cells were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092) and PE Rat Anti-Mouse CD150 (Cat. No. 562651), staining cells well above background compared to PE Rat IgG2a, κ isotype control (Cat. No. 557690) (data not shown). A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by events with the forward and side-light scattering characteristics of viable lymphocytes. (Panel B) BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE-Cy™7 Hamster Anti-Mouse CD48 (Cat. No. 560731), and PE Rat Anti-Mouse CD150 antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P4 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated). Lineage-negative and CD48- CD150+ (Middle Plot, P3 Gate) bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
SLAM; Slamf1; Signaling lymphocytic activation molecule family member 1
Mouse (QC Testing)
Rat IgG2a, κ
Mouse CD150 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
27218
AB_2737705
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554689
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FITC Rat Anti-Mouse CD41 RUO
Size
50 µg
Cat No.
561849
V450 Mouse Lineage Antibody Cocktail, with Isotype Control RUO
Size
100 Tests
Cat No.
561301
PE-Cy™7 Hamster Anti-Mouse CD48 RUO
Size
50 µg
Cat No.
560731
APC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553092
562651 Rev. 4
Antibody Details
Q38-480
The Q38-480 monoclonal antibody specifically binds to mouse CD150, also known as SLAM (signaling lymphocyte activation molecule). CD150 is a type 1 transmembrane glycoprotein that is a member of the CD2 subfamily of the Ig superfamily. It is encoded by the Slamf1 (signaling lymphocytic activation molecule family member 1) gene. CD150 is differentially expressed on subsets of thymocytes, T and B lymphocytes, dendritic cells, macrophages, and endothelial cells. SLAM plays multiple roles in innate and adaptive immunity serving as an adhesion molecule and/or coreceptor. CD150-mediated costimulation of TCR-activated T cells reportedly results in the increased production of IFN-γ by Th1 cells and is required for IL-4 production by T follicular helper cells. CD150 also plays important roles in hematopoietic cell developmental pathways. CD150 is differentially expressed by self-renewing adult hematopoietic stem cells (HSC) whereas non-multipotent hematopoietic progenitor cells are CD150-. Utilizing additional cell surface markers, lineage-negative CD150+CD48-CD41- cell fractions are reported to be highly enriched for adult HSC.
562651 Rev. 4
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562651 Rev.4
Citations & References
Development References (4)
-
Castro AG, Hauser TM, Cocks BG, et al. Molecular and functional characterization of mouse signaling lymphocytic activation molecule (SLAM): differential expression and responsiveness in Th1 and Th2 cells.. J Immunol. 1999; 163(11):5860-70. (Biology). View Reference
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Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005; 121(7):1109-1121. (Biology). View Reference
-
Wang N, Satoskar A, Faubion W, et al. The cell surface receptor SLAM controls T cell and macrophage functions. J Exp Med. 2004; 199(9):1255-1264. (Biology). View Reference
-
Yusuf I, Kageyama R, Monticelli L, et al. Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150). J Immunol. 2010; 185(1):190-202. (Biology). View Reference
562651 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
