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      cDNA Kit

      BD Rhapsody™ cDNA Kit

      (RUO)
      Product Details
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      BD Rhapsody™
      Single cell multiomics experiments on the BD Rhapsody™ platform
      RUO


      Description

      Once the RNA and protein information are captured from single cells on the BD Rhapsody™ Cell Capture Beads, reagents provided in the BD Rhapsody™ cDNA Kit allow you to perform reverse transcription and generate cDNA for use as a template for all your desired downstream library preparations. The kit can be used with the BD Rhapsody™ Targeted Assay, BD Rhapsody™ Whole Transcriptome Analysis Amplification Kit, BD Rhapsody™ TCR/BCR Profiling Assays, BD®  Single-Cell Multiplexing Kit and BD®  AbSeq Assays.Learn more about the intended use of this kit and the associated protocol from the BD Rhapsody™ Single-Cell Analysis System user guide .

      633773 Rev. 1
      Citations & References
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      Development References (28)

      1. Alivernini S, MacDonald L, Elmesmari A, et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020; 26(8):1295-1306. (Biology). View Reference
      2. Arroyo Hornero R, Georgiadis C, Hua P, et al. CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Commun Biol. 2020; 3(1):375. (Biology). View Reference
      3. De Domenico E, Bonaguro L, Schulte-Schrepping J, Becker M, Händler K, Schultze JL. Optimized workflow for single-cell transcriptomics on infectious diseases including COVID-19. STAR Protoc. 2020; 1(3):100233. (Biology). View Reference
      4. Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level. STAR Protoc. 2020; 1(2):100092. (Biology). View Reference
      5. Ferreira RC, Castro Dopico X, Oliveira JJ, et al. Chronic Immune Activation in Systemic Lupus Erythematosus and the Autoimmune PTPN22 Trp620 Risk Allele Drive the Expansion of FOXP3+ Regulatory T Cells and PD-1 Expression. Front Immunol. 2019; 10:2606. (Biology). View Reference
      6. Gertz ML, Chin CR, Tomoiaga D, et al. Multi-omic, Single-Cell, and Biochemical Profiles of Astronauts Guide Pharmacological Strategies for Returning to Gravity. Cell Rep. 2020; 33(10):108429. (Biology). View Reference
      7. Hasegawa T, Kikuta J, Sudo T, et al. Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1. Nat Immunol. 2019; 20(12):1631-1643. (Biology). View Reference
      8. Jensen-Cody SO, Flippo KH, Claflin KE, et al. FGF21 Signals to Glutamatergic Neurons in the Ventromedial Hypothalamus to Suppress Carbohydrate Intake. Cell Metab. 2020; 32(2):273-286. (Biology). View Reference
      9. Li H, Wang H, Sokulsky L, et al. Single-cell transcriptomic analysis reveals key immune cell phenotypes in the lungs of patients with asthma exacerbation. J Allergy Clin Immunol. 2020; S0091(20):31400-7. (Biology). View Reference
      10. Li S, Simoni Y, Becht E, et al. Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer. Cell Rep Med. 2020; 1(3):100039. (Biology). View Reference
      11. Li Z, Li M, Shi SX, et al. Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. J Exp Med. 2020; 217(12):e20200213. (Biology). View Reference
      12. Mair F, Erickson JR, Voillet V, et al. A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell Rep. 2020; 31(1):107499. (Biology). View Reference
      13. Malkani S, Chin CR, Cekanaviciute E, et al. Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development. Cell Rep. 2020; 33(10):108448. (Biology). View Reference
      14. Mohebiany AN, Ramphal NS, Karram K, et al. Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology. Cell Rep. 2020; 30(5):1585-1597. (Biology). View Reference
      15. Sainson RCA, Thotakura AK, Kosmac M, et al. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression. Cancer Immunol Res. 2020; 8(12):1568-1582. (Biology). View Reference
      16. Schulte-Schrepping J, Reusch N, Paclik D, et al. Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. Cell. 2020; 182(6):1419-1440. (Biology). View Reference
      17. Song B, Xiong G, Luo H, Zuo Z, Zhou Z, Chang X. Single-cell RNA sequencing of mouse neural stem cell differentiation reveals adverse effects of cadmium on neurogenesis. Food Chem Toxicol. 2021; (148):111936. (Biology). View Reference
      18. Song L, Zhang S, Yu S, et al. Cellular heterogeneity landscape in laryngeal squamous cell carcinoma. Int J Cancer. Int J Cancer. 2020; 147(10):2879-2890. (Biology). View Reference
      19. Sun H, Wen X, Li H, et al. Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration. Ann Rheum Dis. 2020; 79(3):408-417. (Biology). View Reference
      20. Tang X, Zhang S, Peng Q, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020; 6(8):eaaz0374. (Biology). View Reference
      21. Trzupek D, Dunstan M, Cutler AJ, et al. Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis. Genome Med. 2020; 12(1):55. (Biology). View Reference
      22. Wagar LE, Salahudeen A, Constantz CM, et al. Modeling human adaptive immune responses with tonsil organoids. Nat Med.. 2021; 27(1):125-135. (Biology). View Reference
      23. Wen M, Cai G, Ye J, Liu X, Ding H, Zeng H. Single-cell transcriptomics reveals the alteration of peripheral blood mononuclear cells driven by sepsis. Ann Transl Med. 2020; 8(4):125. (Biology). View Reference
      24. Wu B, Wang W, Wang H, et al. Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2. Front Oncol. 2020; (10):566599. (Biology). View Reference
      25. Wu M, Wang C, Shi B, et al. A novel recombinant cccDNA-based mouse model with long term maintenance of rcccDNA and antigenemia. Antiviral Res. 2020; 180:104826. (Biology). View Reference
      26. Xie X, Shi Q, Wu P, et al. Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection.. Nat Immunol. 2020; 21(9):1119-1133. (Biology). View Reference
      27. Yang Y, Liu F, Liu W, et al. Analysis of single-cell RNAseq identifies transitional states of T cells associated with hepatocellular carcinoma. Clin Transl Med. 2020; 10(3):e133. (Biology). View Reference
      28. Zhao L, Yao C, Xing X, et al. Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells. Nat Commun. 2020; 11(1):5683. (Biology). View Reference
      View All (28) View Less
      633773 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.